AJP - Endocrinology and Metabolism, Vol 267, Issue 4 E489-E496, Copyright © 1994 by American Physiological Society

ARTICLES

Release of gastric inhibitory polypeptide from cultured canine endocrine cells

T. J. Kieffer, A. M. Buchan, H. Barker, J. C. Brown and R. A. Pederson
Department of Physiology, University of British Columbia, Vancouver, Canada.

Canine intestinal duodenal and jejunal epithelial cell preparations enriched for endocrine cells were obtained by sequential collagenase digestion and centrifugal elutriation and maintained in culture for a 40-h period. Adherent cells contained a total cell content (TCC) of 11.5 +/- 2.5 ng (mean +/- SE) immunoreactive gastric inhibitory peptide (IRGIP)/well and 1.4 +/- 0.2 ng immunoreactive somatostatin (IRS)/well. Release experiments were performed by incubation of the cells with various stimuli over a 2-h period. Basal release of IRGIP in 5 mM glucose-5 mM K+ was 2.7 +/- 0.4% TCC. Incubation with concentrations of K+ > 20 mM or glucose > 15 mM significantly increased IRGIP release, as did the addition of a somatostatin immunoneutralizing antibody to the basal media. The addition of the Ca2+ ionophore, A-23187 (10 microM), or the adenylate cyclase activator, forskolin (100 microM), resulted in an IRGIP output greater than four times basal. Porcine gastrin-releasing peptide (GRP), at 1-100 nM, significantly stimulated IRGIP release in a concentration-dependent fashion. IRS release was increased significantly by 55 mM K+, 20 mM glucose, 10 microM A-23187, 100 nM GRP, or 100 microM forskolin.

This article has been cited by other articles:

				Y. Fujita, J. W. Y. Chui, D. S. King, T. Zhang, J. Seufert, S. Pownall, A. T. Cheung, and T. J. Kieffer Pax6 and Pdx1 are required for production of glucose-dependent insulinotropic polypeptide in proglucagon-expressing L cells Am J Physiol Endocrinol Metab, September 1, 2008; 295(3): E648 - E657. [Abstract] [Full Text] [PDF]

				L. Li and B. M. Wice Bombesin and nutrients independently and additively regulate hormone release from GIP/Ins cells Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E208 - E215. [Abstract] [Full Text] [PDF]

				F. C. Schuit, P. Huypens, H. Heimberg, and D. G. Pipeleers Glucose Sensing in Pancreatic {beta}-Cells: A Model for the Study of Other Glucose-Regulated Cells in Gut, Pancreas, and Hypothalamus Diabetes, January 1, 2001; 50(1): 1 - 11. [Abstract] [Full Text]

				A. T. Cheung, B. Dayanandan, J. T. Lewis, G. S. Korbutt, R. V. Rajotte, M. Bryer-Ash, M. O. Boylan, M. M. Wolfe, and T. J. Kieffer Glucose-Dependent Insulin Release from Genetically Engineered K Cells Science, December 8, 2000; 290(5498): 1959 - 1962. [Abstract] [Full Text]

				A. M. J. Buchan Nutrient Tasting and Signaling Mechanisms in the Gut. III. Endocrine cell recognition of luminal nutrients Am J Physiol Gastrointest Liver Physiol, December 1, 1999; 277(6): G1103 - G1107. [Abstract] [Full Text] [PDF]

				M. O. Boylan, L. I. Jepeal, L. A. Jarboe, and M. M. Wolfe Cell-specific Expression of the Glucose-dependent Insulinotropic Polypeptide Gene in a Mouse Neuroendocrine Tumor Cell Line J. Biol. Chem., July 11, 1997; 272(28): 17438 - 17443. [Abstract] [Full Text] [PDF]