AJP - Endocrinology and Metabolism, Vol 267, Issue 3 E461-E466, Copyright © 1994 by American Physiological Society

ARTICLES

Comparative effects of IGF-I and insulin on the glucose transporter system in rat muscle

S. Lund, A. Flyvbjerg, G. D. Holman, F. S. Larsen, O. Pedersen and O. Schmitz
Department of Biochemistry, University of Bath, United Kingdom.

The acute effect of insulin-like growth factor I (IGF-I) and insulin on glucose uptake and the glucose transport system in in vitro incubated rat soleus muscles was examined using 3-O-methylglucose and the ATB-[3H]BMPA exofacial photolabeling technique. IGF-I and insulin both stimulated 3-O-methylglucose uptake and GLUT-4 translocation in a dose-dependent manner with a maximal effect six- to sevenfold above basal. No additive effects of IGF-I and insulin on maximal 3-O-methylglucose uptake were found. On a molar basis, IGF-I was 13 times less potent than insulin. Receptor binding experiments showed that IGF-I exhibited a much lower affinity for the insulin receptor [half-maximal effective dose (ED50) = 28.5 nM] than that of insulin (ED50 = 0.20 nM). In contrast, IGF-I bound to the partially purified IGF-I receptor with an apparent affinity (ED50 = 3.7 nM) that was similar to the concentrations of IGF-I which caused half-maximal activation of 3-O-methylglucose uptake (ED50 = 2.4 nM) and GLUT-4 translocation (ED50 = 2.5 nM). Our findings suggest that IGF-I exerts its insulin-like effects on glucose uptake primarily through its own specific receptor and that the molecular events underlying IGF-I and insulin actions on glucose uptake in skeletal muscle are similar, namely caused by a translocation of the GLUT-4 transporter from an intracellular pool to the cell surface.

This article has been cited by other articles:

				N. Jessen, C. B. Djurhuus, J. O. L. Jorgensen, L. S. Jensen, N. Moller, S. Lund, and O. Schmitz Evidence against a role for insulin-signaling proteins PI 3-kinase and Akt in insulin resistance in human skeletal muscle induced by short-term GH infusion Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E194 - E199. [Abstract] [Full Text] [PDF]

				C. L. Dumke, J. S. Rhodes, T. Garland Jr, E. Maslowski, J. G. Swallow, A. C. Wetter, and G. D. Cartee Genetic selection of mice for high voluntary wheel running: effect on skeletal muscle glucose uptake J Appl Physiol, September 1, 2001; 91(3): 1289 - 1297. [Abstract] [Full Text] [PDF]

				E. S. Buhl, N. Jessen, O. Schmitz, S. B. Pedersen, O. Pedersen, G. D. Holman, and S. Lund Chronic Treatment With 5-Aminoimidazole-4-Carboxamide-1-{beta}-D-Ribofuranoside Increases Insulin-Stimulated Glucose Uptake and GLUT4 Translocation in Rat Skeletal Muscles in a Fiber Type--Specific Manner Diabetes, January 1, 2001; 50(1): 12 - 17. [Abstract] [Full Text]

				W. Derave, B. F. Hansen, S. Lund, S. Kristiansen, and E. A. Richter Muscle glycogen content affects insulin-stimulated glucose transport and protein kinase B activity Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E947 - E955. [Abstract] [Full Text] [PDF]

				J. W. RYDER, Y. KAWANO, D. GALUSKA, R. FAHLMAN, H. WALLBERG-HENRIKSSON, M. J. CHARRON, and J. R. ZIERATH Postexercise glucose uptake and glycogen synthesis in skeletal muscle from GLUT4-deficient mice FASEB J, December 1, 1999; 13(15): 2246 - 2256. [Abstract] [Full Text]

				P. R. Shepherd and B. B. Kahn Glucose Transporters and Insulin Action -- Implications for Insulin Resistance and Diabetes Mellitus N. Engl. J. Med., July 22, 1999; 341(4): 248 - 257. [Full Text] [PDF]

				D. J. Dean, J. T. Brozinick Jr., S. W. Cushman, and G. D. Cartee Calorie restriction increases cell surface GLUT-4 in insulin-stimulated skeletal muscle Am J Physiol Endocrinol Metab, December 1, 1998; 275(6): E957 - E964. [Abstract] [Full Text] [PDF]

				J. R. Zierath, T.-S. Tsao, A. E. Stenbit, J. W. Ryder, D. Galuska, and M. J. Charron Restoration of Hypoxia-stimulated Glucose Uptake in GLUT4-deficient Muscles by Muscle-specific GLUT4 Transgenic Complementation J. Biol. Chem., August 14, 1998; 273(33): 20910 - 20915. [Abstract] [Full Text] [PDF]

				M. Pendergrass, E. Fazioni, D. Collins, and R. A. DeFronzo IGF-I increases forearm blood flow without increasing forearm glucose uptake Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E345 - E350. [Abstract] [Full Text] [PDF]

				J. Y. Hokama, R. S. Streeper, and E. J. Henriksen Voluntary exercise training enhances glucose transport in muscle stimulated by insulin-like growth factor I J Appl Physiol, February 1, 1997; 82(2): 508 - 512. [Abstract] [Full Text] [PDF]