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Am J Physiol Endocrinol Metab 267: E132-E139, 1994;
0193-1849/94 $5.00
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AJP - Endocrinology and Metabolism, Vol 267, Issue 1 E132-E139, Copyright © 1994 by American Physiological Society

ARTICLES

GLUT-4 degradation rate: reduction in rat adipocytes in fasting and streptozotocin-induced diabetes

S. S. Kim, J. W. Bae and C. Y. Jung
Biophysical Laboratory, Veterans Administration Medical Center, Buffalo, New York.

With the use of [3H]leucine pulse-chase and immunoprecipitation methods, we measured the rate of GLUT-4 degradation in rat adipocytes in the steady state at 37 degrees C. We also studied the relationship of the reduced GLUT-4 levels observed in fasted and streptozotocin (STZ)-induced diabetic rats on degradation. GLUT-4 degradation was a simple, first-order decay process. The decay was describable by a single, first-order rate constant (k). A k value of 0.061/h was estimated in control rat adipocytes. In the adipocytes of fasted and STZ-induced diabetic rats, cellular GLUT-4 contents were reduced to 36 and 43% of the control, respectively. The rates of GLUT-4 degradation were also reduced significantly, with kappa values of 0.038 and 0.041/h, respectively. These changes were reversible; the decreased values returned to control values when GLUT-4 contents were normalized by refeeding and insulin injection. These findings demonstrate the presence of a posttranslational mechanism in rat adipocytes that reduces the GLUT-4 degradation rate constant when the cellular GLUT-4 level is reduced by a pretranslational defect.


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