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AJP - Endocrinology and Metabolism, Vol 266, Issue 2 E193-E201, Copyright © 1994 by American Physiological Society
ARTICLES |
T. C. Vary, C. V. Jurasinski, A. M. Karinch and S. R. Kimball
Department of Cellular and Molecular Physiology, Pennsylvania State University, College of Medicine, Hershey 17033.
Protein synthesis is stimulated at the level of peptide chain initiation in livers from rats with a sterile or septic abscess. In contrast, peptide chain initiation is inhibited in fast-twitch skeletal muscles from septic rats. We investigated the possible mechanisms responsible for these differential changes in peptide chain initiation between liver and skeletal muscle during sepsis by measuring the cellular content of eukaryotic initiation factor-2 (eIF-2), the extent of phosphorylation of the alpha-subunit of eIF-2, and the activity of eIF-2B. In skeletal muscle, neither the eIF-2 content nor the extent of phosphorylation of eIF-2 alpha was altered during sepsis. However, a significant decrease (P < 0.001) in eIF-2B activity was observed in fast-twitch muscles. In liver, neither the extent of phosphorylation of eIF-2 alpha nor the activity of eIF-2B was different in rats with a sterile or septic abscess compared with control. However, the amount of eIF-2 in liver was increased in both sterile inflammation and sepsis. The relative abundance of eIF-2 alpha mRNA was not increased in either condition compared with control. Analysis of the distribution of eIF-2 alpha mRNA from control rats revealed that only approximately 40% of the message was associated with polysomes. Sterile inflammation or sepsis caused a 50% increase in the proportion of eIF-2 alpha mRNA associated with the polysomes compared with control.(ABSTRACT TRUNCATED AT 250 WORDS)
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 T. C. Vary, S. R. Kimball, and A. Sumner Sex-dependent differences in the regulation of myocardial protein synthesis following long-term ethanol consumption Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2007; 292(2): R778 - R787. [Abstract] [Full Text] [PDF]
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R. A. Orellana, S. R. Kimball, A. Suryawan, J. Escobar, H. V. Nguyen, L. S. Jefferson, and T. A. Davis Insulin stimulates muscle protein synthesis in neonates during endotoxemia despite repression of translation initiation Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E629 - E636. [Abstract] [Full Text] [PDF]
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C. H. Lang, R. A. Frost, and T. C. Vary Thermal injury impairs cardiac protein synthesis and is associated with alterations in translation initiation Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2004; 286(4): R740 - R750. [Abstract] [Full Text] [PDF]
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S. R. Kimball, R. A. Orellana, P. M. J. O'Connor, A. Suryawan, J. A. Bush, H. V. Nguyen, M. C. Thivierge, L. S. Jefferson, and T. A. Davis Endotoxin induces differential regulation of mTOR-dependent signaling in skeletal muscle and liver of neonatal pigs Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E637 - E644. [Abstract] [Full Text] [PDF]
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T. C. Vary, G. Deiter, and S. R. Kimball Phosphorylation of eukaryotic initiation factor eIF2Bepsilon in skeletal muscle during sepsis Am J Physiol Endocrinol Metab, November 1, 2002; 283(5): E1032 - E1039. [Abstract] [Full Text] [PDF]
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T. C. Vary, L. S. Jefferson, and S. R. Kimball Insulin fails to stimulate muscle protein synthesis in sepsis despite unimpaired signaling to 4E-BP1 and S6K1 Am J Physiol Endocrinol Metab, November 1, 2001; 281(5): E1045 - E1053. [Abstract] [Full Text] [PDF]
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C. H. Lang, R. A. Frost, V. Kumar, and T. C. Vary Impaired myocardial protein synthesis induced by acute alcohol intoxication is associated with changes in eIF4F Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1029 - E1038. [Abstract] [Full Text] [PDF]
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T. C. Vary and S. R. Kimball Effect of sepsis on eIE4E availability in skeletal muscle Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1178 - E1184. [Abstract] [Full Text] [PDF]
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C. H. Lang, R. A. Frost, L. S. Jefferson, S. R. Kimball, and T. C. Vary Endotoxin-induced decrease in muscle protein synthesis is associated with changes in eIF2B, eIF4E, and IGF-I Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E1133 - E1143. [Abstract] [Full Text] [PDF]
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C. H. Lang, D. Wu, R. A. Frost, L. S. Jefferson, T. C. Vary, and S. R. Kimball Chronic alcohol feeding impairs hepatic translation initiation by modulating eIF2 and eIF4E Am J Physiol Endocrinol Metab, November 1, 1999; 277(5): E805 - E814. [Abstract] [Full Text] [PDF]
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C. H. Lang, D. Wu, R. A. Frost, L. S. Jefferson, S. R. Kimball, and T. C. Vary Inhibition of muscle protein synthesis by alcohol is associated with modulation of eIF2B and eIF4E Am J Physiol Endocrinol Metab, August 1, 1999; 277(2): E268 - E276. [Abstract] [Full Text] [PDF]
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R. Cooney, S. R. Kimball, R. Eckman, G. Maish III, M. Shumate, and T. C. Vary TNF-binding protein ameliorates inhibition of skeletal muscle protein synthesis during sepsis Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E611 - E619. [Abstract] [Full Text] [PDF]
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