AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 265: E259-E266, 1993;
0193-1849/93 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Giddings, S. J.
Right arrow Articles by Shalwitz, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Giddings, S. J.
Right arrow Articles by Shalwitz, R. A.

AJP - Endocrinology and Metabolism, Vol 265, Issue 2 E259-E266, Copyright © 1993 by American Physiological Society

ARTICLES

Hypoglycemia but not hyperglycemia induces rapid changes in pancreatic beta-cell gene transcription

S. J. Giddings, L. R. Carnaghi and R. A. Shalwitz
Research Service, St. Louis Veterans Affairs Medical Center, Missouri 63106.

The purpose of these studies was to quantify several mRNAs expressed specifically in pancreatic islet cells and known or postulated to be important for insulin release after acute well defined alterations in levels of plasma glucose. Glucose levels were maintained at 50, 120, or 180 mg/dl (2.8, 6.7, or 10 mM) for 3 h in conscious unrestrained rats. Hypoglycemia (for 3 h) caused significant decreases in pancreatic content of mRNAs for insulin 2 and GLUT-2 to 55 and 34% of control values, respectively. There were no significant changes in insulin 1, amylin, glucokinase, or glucagon mRNAs. Unprocessed insulin 1 and 2 mRNA precursors were decreased to 17 and 10% of levels in controls, consistent with effects of short-term hypoglycemia on new mRNA synthesis. Hyperglycemia (for 3 h) caused no increase in pancreatic content of any mRNA measured. To discriminate between effects of hypoglycemia and hyperinsulinemia in the hypoglycemic animals, rats were made hypoglycemic by infusion with etomoxir, a carnitine palmitoyltransferase I inhibitor that lowers glucose in the fasted (glycogen-depleted) state by inhibiting hepatic gluconeogenesis. A single dose of this agent caused a decrease in glucose from 120 mg/dl (6.7 mM) to 80 mg/dl (4.4 mM) and significantly decreased insulin mRNA and pre-mRNA. These results are consistent with the hypothesis that glucose modulates islet cell gene transcription directly. They indicate that the range of glucose concentrations that modulate gene transcription differs from the levels of glucose that alter both insulin biosynthetic and secretion rates.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. M. Steil, N. Trivedi, J.-C. Jonas, W. M. Hasenkamp, A. Sharma, S. Bonner-Weir, and G. C. Weir
Adaptation of {beta}-cell mass to substrate oversupply: enhanced function with normal gene expression
Am J Physiol Endocrinol Metab, May 1, 2001; 280(5): E788 - E796.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Leibiger, T. Moede, T. Schwarz, G. R. Brown, M. Kohler, I. B. Leibiger, and P.-O. Berggren
Short-term regulation of insulin gene transcription by glucose
PNAS, August 4, 1998; 95(16): 9307 - 9312.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. M. Macfarlane, S. C. Campbell, L. J. Elrick, V. Oates, G. Bermano, K. J. Lindley, A. Aynsley-Green, M. J. Dunne, R. F. L. James, and K. Docherty
Glucose Regulates Islet Amyloid Polypeptide Gene Transcription in a PDX1- and Calcium-dependent Manner
J. Biol. Chem., May 12, 2000; 275(20): 15330 - 15335.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online