| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Endocrinology and Metabolism, Vol 264, Issue 5 E693-E698, Copyright © 1993 by American Physiological Society
ARTICLES |
S. Welle, C. Thornton, R. Jozefowicz and M. Statt
Department of Medicine, University of Rochester, New York 14620.
We tested the hypothesis that healthy older men (> 60 yr old) have a slower rate of myofibrillar protein synthesis than young men (< 35 yr old). Myofibrillar protein synthesis was determined by the in vivo incorporation of L-[1-13C]leucine into myofibrillar proteins obtained by muscle biopsy. Subjects were eight young (21-31 yr) and eight older (62-81 yr) men, all healthy and moderately active. There was no significant difference in the mean height and weight of the two age groups, but the older group had 12% less lean body mass (determined by 40K counting) and 21% less muscle mass (estimated by urinary creatinine excretion). Upper leg strength was approximately one-third lower in the older subjects according to isokinetic dynamometry. The fractional rate of myofibrillar protein synthesis was 28% slower in the older group (0.039 +/- 0.009 vs. 0.054 +/- 0.010 %/h, mean +/- SD, P < 0.01). Total myofibrillar protein synthesis, estimated as total myofibrillar mass (from creatinine excretion) times the fractional synthesis rate, was 44% slower in the older group (1.4 vs. 2.5 g/h, P < 0.001). Whole body protein synthesis, assessed as the difference between leucine disappearance rate and leucine oxidation, was marginally slower (8%, P = 0.10) in the older group, but not when the data were adjusted for lean body mass. Myofibrillar protein synthesis was a smaller fraction of whole body protein synthesis in the older group (12 vs. 19%). Reduced myofibrillar protein synthesis may be an important mechanism of the muscle atrophy associated with aging.
This article has been cited by other articles:
|
|
 |
|
  L. V. Thompson, D. Durand, N. A. Fugere, and D. A. Ferrington Myosin and actin expression and oxidation in aging muscle J Appl Physiol, December 1, 2006; 101(6): 1581 - 1587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Haddad and G. R. Adams Aging-sensitive cellular and molecular mechanisms associated with skeletal muscle hypertrophy J Appl Physiol, April 1, 2006; 100(4): 1188 - 1203. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Welle, K. Bhatt, and C. A. Pinkert Myofibrillar protein synthesis in myostatin-deficient mice Am J Physiol Endocrinol Metab, March 1, 2006; 290(3): E409 - E415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Balagopal, R. Olney, D. Darmaun, E. Mougey, M. Dokler, G. Sieck, and D. Hammond Oxandrolone enhances skeletal muscle myosin synthesis and alters global gene expression profile in Duchenne muscular dystrophy Am J Physiol Endocrinol Metab, March 1, 2006; 290(3): E530 - E539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Toth, D. E. Matthews, R. P. Tracy, and M. J. Previs Age-related differences in skeletal muscle protein synthesis: relation to markers of immune activation Am J Physiol Endocrinol Metab, May 1, 2005; 288(5): E883 - E891. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Thomson and S. E. Gordon Diminished overload-induced hypertrophy in aged fast-twitch skeletal muscle is associated with AMPK hyperphosphorylation J Appl Physiol, February 1, 2005; 98(2): 557 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Kimball, J. P. O'Malley, J. C. Anthony, S. J. Crozier, and L. S. Jefferson Assessment of biomarkers of protein anabolism in skeletal muscle during the life span of the rat: sarcopenia despite elevated protein synthesis Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E772 - E780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Parkington, N. K. LeBrasseur, A. P. Siebert, and R. A. Fielding Contraction-mediated mTOR, p70S6k, and ERK1/2 phosphorylation in aged skeletal muscle J Appl Physiol, July 1, 2004; 97(1): 243 - 248. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Paddon-Jones, M. Sheffield-Moore, X.-J. Zhang, E. Volpi, S. E. Wolf, A. Aarsland, A. A. Ferrando, and R. R. Wolfe Amino acid ingestion improves muscle protein synthesis in the young and elderly Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E321 - E328. [Abstract] [Full Text]
|
|
|
|
|
|
K. R. Short, J. L. Vittone, M. L. Bigelow, D. N. Proctor, and K. S. Nair Age and aerobic exercise training effects on whole body and muscle protein metabolism Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E92 - E101. [Abstract] [Full Text]
|
|
|
|
|
|
A. M. Payne, S. L. Dodd, and C. Leeuwenburgh Life-long calorie restriction in Fischer 344 rats attenuates age-related loss in skeletal muscle-specific force and reduces extracellular space J Appl Physiol, December 1, 2003; 95(6): 2554 - 2562. [Abstract] [Full Text]
|
|
|
|
|
|
F. Lauretani, C. R. Russo, S. Bandinelli, B. Bartali, C. Cavazzini, A. Di Iorio, A. M. Corsi, T. Rantanen, J. M. Guralnik, and L. Ferrucci Age-associated changes in skeletal muscles and their effect on mobility: an operational diagnosis of sarcopenia J Appl Physiol, November 1, 2003; 95(5): 1851 - 1860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Roubenoff Sarcopenia: Effects on Body Composition and Function J. Gerontol. A Biol. Sci. Med. Sci., November 1, 2003; 58(11): M1012 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Doherty Invited Review: Aging and sarcopenia J Appl Physiol, October 1, 2003; 95(4): 1717 - 1727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Welle, A. I. Brooks, J. M. Delehanty, N. Needler, and C. A. Thornton Gene expression profile of aging in human muscle Physiol Genomics, July 7, 2003; 14(2): 149 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. O. Marx, W. J. Kraemer, B. C. Nindl, and L. Larsson Effects of Aging on Human Skeletal Muscle Myosin Heavy-Chain mRNA Content and Protein Isoform Expression J. Gerontol. A Biol. Sci. Med. Sci., June 1, 2002; 57(6): B232 - 238. [Abstract] [Full Text]
|
|
|
|
|
|
S. A. Jubrias, P. C. Esselman, L. B. Price, M. E. Cress, and K. E. Conley Large energetic adaptations of elderly muscle to resistance and endurance training J Appl Physiol, May 1, 2001; 90(5): 1663 - 1670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. WELLE, A. BROOKS, and C. A. THORNTON Senescence-related changes in gene expression in muscle: similarities and differences between mice and men Physiol Genomics, March 8, 2001; 5(2): 67 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Balagopal, J. C. Schimke, P. Ades, D. Adey, and K. S. Nair Age effect on transcript levels and synthesis rate of muscle MHC and response to resistance exercise Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E203 - E208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. W.R. Lemon Beyond the Zone: Protein Needs of Active Individuals J. Am. Coll. Nutr., October 1, 2000; 19(90005): 513S - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. ZAINAL, T. D. OBERLEY, D. B. ALLISON, L. I. SZWEDA, and R. WEINDRUCH Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle FASEB J, September 1, 2000; 14(12): 1825 - 1836. [Abstract] [Full Text]
|
|
|
|
|
|
D. L. Hasten, J. Pak-Loduca, K. A. Obert, and K. E. Yarasheski Resistance exercise acutely increases MHC and mixed muscle protein synthesis rates in 78-84 and 23-32 yr olds Am J Physiol Endocrinol Metab, April 1, 2000; 278(4): E620 - E626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Morais, R. Ross, R. Gougeon, P. B. Pencharz, P. J. H. Jones, and E. B. Marliss Distribution of Protein Turnover Changes with Age in Humans as Assessed by Whole-Body Magnetic Resonance Image Analysis to Quantify Tissue Volumes J. Nutr., April 1, 2000; 130(4): 784 - 791. [Abstract] [Full Text]
|
|
|
|
|
|
L. Mosoni, T. Malmezat, M. C. Valluy, M. L. Houlier, D. Attaix, and P. P. Mirand Lower recovery of muscle protein lost during starvation in old rats despite a stimulation of protein synthesis Am J Physiol Endocrinol Metab, October 1, 1999; 277(4): E608 - E616. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Volpi, B. Mittendorfer, S. E. Wolf, and R. R. Wolfe Oral amino acids stimulate muscle protein anabolism in the elderly despite higher first-pass splanchnic extraction Am J Physiol Endocrinol Metab, September 1, 1999; 277(3): E513 - E520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Yarasheski, J. Pak-Loduca, D. L. Hasten, K. A. Obert, M. B. Brown, and D. R. Sinacore Resistance exercise training increases mixed muscle protein synthesis rate in frail women and men >= 76 yr old Am J Physiol Endocrinol Metab, July 1, 1999; 277(1): E118 - E125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-A. Arnal, L. Mosoni, Y. Boirie, M.-L. Houlier, L. Morin, E. Verdier, P. Ritz, J.-M. Antoine, J. Prugnaud, B. Beaufrere, et al. Protein pulse feeding improves protein retention in elderly women Am. J. Clinical Nutrition, June 1, 1999; 69(6): 1202 - 1208. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease . A Statement of the American Thoracic Society and European Respiratory Society Am. J. Respir. Crit. Care Med., April 1, 1999; 159(4): S2 - 40. [Full Text] [PDF]
|
|
|
|
|
|
N. C. Jackson, P. V. Carroll, D. L. Russell-Jones, P. H. Sonksen, D. F. Treacher, and A. M. Umpleby The metabolic consequences of critical illness: acute effects on glutamine and protein metabolism Am J Physiol Endocrinol Metab, January 1, 1999; 276(1): E163 - E170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Piers, M. J. Soares, L. M. McCormack, and K. O'Dea Is there evidence for an age-related reduction in metabolic rate? J Appl Physiol, December 1, 1998; 85(6): 2196 - 2204. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Welle and C. A. Thornton High-protein meals do not enhance myofibrillar synthesis after resistance exercise in 62- to 75-yr-old men and women Am J Physiol Endocrinol Metab, April 1, 1998; 274(4): E677 - E683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Proctor, P. Balagopal, and K. S. Nair Age-Related Sarcopenia in Humans Is Associated with Reduced Synthetic Rates of Specific Muscle Proteins J. Nutr., February 1, 1998; 128(2): 351 - 351. [Abstract] [Full Text]
|
|
|
|
|
|
P. Balagopal, O. E. Rooyackers, D. B. Adey, P. A. Ades, and K. S. Nair Effects of aging on in vivo synthesis of skeletal muscle myosin heavy-chain and sarcoplasmic protein in humans Am J Physiol Endocrinol Metab, October 1, 1997; 273(4): E790 - E800. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. E. Rooyackers, D. B. Adey, P. A. Ades, and K. S. Nair Effect of age on in vivo rates of mitochondrial protein synthesis in human skeletal muscle PNAS, December 24, 1996; 93(26): 15364 - 15369. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
