AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 263: E724-E729, 1992;
0193-1849/92 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Turner, R. T.
Right arrow Articles by Spelsberg, T. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Turner, R. T.
Right arrow Articles by Spelsberg, T. C.

AJP - Endocrinology and Metabolism, Vol 263, Issue 4 E724-E729, Copyright © 1992 by American Physiological Society

ARTICLES

Tissue-specific expression of bone proteins in femora of growing rats

R. T. Turner, S. N. Kapelner and T. C. Spelsberg
Department of Orthopedic Surgery, Mayo Clinic, Rochester, Minnesota 55905.

Total cellular RNA was extracted from bone cells of three different femoral compartments of 2-mo-old rats. The intact femora were first incubated with collagenase to obtain periosteal cells. The bisected periosteum-free diaphyses and metaphyses were then incubated with collagenase to obtain enriched populations of endosteal and cancellous bone cells, respectively. The total cellular RNA from these three tissues was separated by size using agarose gel electrophoresis, transferred to nylon filters, hybridized to 32P-labeled cDNA probes for glyceraldehyde-3-phosphate dehydrogenase (GAP), pre-pro-alpha (I) type I collagen (collagen), osteocalcin (BGP), and alkaline phosphatase (AP), and the cDNA/mRNA hybrids were visualized by radioautography. Bone matrix deposition was measured in each tissue compartment by tetracycline-based dynamic bone histomorphometry. The bone formation and apposition rates were greatest in the periosteum and least in metaphysis. Mean mRNA levels for collagen and BGP were positively correlated with mean bone formation and mineral apposition rates. Interestingly, mean AP mRNA levels were not correlated with indexes of bone formation. These results demonstrate that the steady-state mRNA levels for bone matrix proteins in femora show pronounced site specificity and correlate with the rates of bone matrix deposition.


This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
G. L. Evans, E. Morey-Holton, and R. T. Turner
Spaceflight has compartment- and gene-specific effects on mRNA levels for bone matrix proteins in rat femur
J Appl Physiol, June 1, 1998; 84(6): 2132 - 2137.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
K. C. Westerlind, J. D. Fluckey, S. E. Gordon, W. J. Kraemer, P. A. Farrell, and R. T. Turner
Effect of resistance exercise training on cortical and cancellous bone in mature male rats
J Appl Physiol, February 1, 1998; 84(2): 459 - 464.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online