AJP - Endocrinology and Metabolism, Vol 263, Issue 3 E500-E506, Copyright © 1992 by American Physiological Society

ARTICLES

Regulation of expression of the lipoprotein lipase gene in brown adipose tissue

J. R. Mitchell, A. Jacobsson, T. G. Kirchgessner, M. C. Schotz, B. Cannon and J. Nedergaard
Wenner-Gren Institute, Arrhenius Laboratories F3, Stockholm University, Sweden.

The regulation of lipoprotein lipase gene expression in brown adipose tissue was studied. Rats were preacclimated to 21 degrees C. Exposure to cold (4 degrees C) resulted in a rapid increase in the level of lipoprotein lipase mRNA in the tissue. The level peaked (expressed per microgram total RNA) after approximately 8 h and then slowly declined. The increased lipoprotein lipase mRNA level was not due to an increased stability of the mRNA, but, in a transition event from a high to a low expression of the lipoprotein lipase gene, a transcription-dependent process was recruited that accelerated the breakdown of lipoprotein lipase mRNA. Norepinephrine injections increased lipoprotein lipase mRNA levels in the tissue; this effect was mediated via a beta-adrenergic receptor. The effect of cold could be mimicked by norepinephrine injections, and these two effects were not additive, indicating that the cold effect was mediated by norepinephrine. The lipoprotein lipase mRNA level was also increased by insulin injections (into fasted animals); thus an increase in lipoprotein lipase gene expression in brown adipose tissue may be induced via two different stimuli, which, intracellularly, would be mediated via different signaling systems. In all investigated conditions, the changes in lipoprotein lipase mRNA levels observed here were parallelled by alterations in lipoprotein lipase activity reported earlier from this laboratory. It was therefore concluded that, under the conditions studied, lipoprotein lipase activity in brown adipose tissue was primarily regulated at the transcriptional level.

This article has been cited by other articles:

				B. CANNON and J. NEDERGAARD Brown Adipose Tissue: Function and Physiological Significance Physiol Rev, January 1, 2004; 84(1): 277 - 359. [Abstract] [Full Text] [PDF]

				M. Bergo, G. Wu, T. Ruge, and T. Olivecrona Down-regulation of Adipose Tissue Lipoprotein Lipase during Fasting Requires That a Gene, Separate from the Lipase Gene, Is Switched On J. Biol. Chem., March 29, 2002; 277(14): 11927 - 11932. [Abstract] [Full Text] [PDF]

				H. M. Guardiola-Diaz, S. Rehnmark, N. Usuda, T. Albrektsen, D. Feltkamp, J.-A. Gustafsson, and S. E. H. Alexson Rat Peroxisome Proliferator-activated Receptors and Brown Adipose Tissue Function during Cold Acclimatization J. Biol. Chem., August 13, 1999; 274(33): 23368 - 23377. [Abstract] [Full Text] [PDF]

				S. Takagi, Y. Kihara, S. Sasayama, and T. Mitsuiye Slow inactivation of cardiac L-type Ca2+ channel induced by cold acclimation of guinea pig Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1998; 274(2): R348 - R356. [Abstract] [Full Text] [PDF]

				P. Tvrdik, A. Asadi, L. P. Kozak, J. Nedergaard, B. Cannon, and A. Jacobsson Cig30, a Mouse Member of a Novel Membrane Protein Gene Family, Is Involved in the Recruitment of Brown Adipose Tissue J. Biol. Chem., December 12, 1997; 272(50): 31738 - 31746. [Abstract] [Full Text] [PDF]