AJP - Endocrinology and Metabolism, Vol 263, Issue 3 E467-E472, Copyright © 1992 by American Physiological Society

ARTICLES

Effect of long-term rhGH administration in GH-deficient adults on fat cell epinephrine response

M. Beauville, I. Harant, F. Crampes, D. Riviere, M. T. Tauber, J. P. Tauber and M. Garrigues
Laboratoire de Physiologie, Faculte de Medecine Toulouse-Purpan, France.

Besides exerting its own lipolytic effect, growth hormone (GH) has been reported to potentiate the lipolytic response of adipose tissue to epinephrine. It was thought interesting to find out whether long-term recombinant human growth hormone (rhGH) administration modifies epinephrine-induced lipolysis in isolated adipocytes of GH-deficient adults. In a double-blind protocol, GH-deficient subjects received either 6 mo placebo (controls, n = 5) or 6 mo rhGH (treated, n = 5). Biopsies of fat were obtained from the periumbilical region before and after placebo or rhGH administration. The response of the collagenase-isolated fat cells to various concentrations of epinephrine was assessed by glycerol release, measured by bioluminescence. Epinephrine-induced lipolysis was not altered by 6 mo placebo, while it was significantly increased by 6 mo rhGH. A similar response was obtained with isoproterenol, but no significant differences occurred in either group with UK 14304, an alpha 2-adrenoreceptor agonist. Thus, in GH-deficient adults, long-term rhGH administration improves the lipolytic response of isolated adipocytes to epinephrine, essentially by increasing the efficiency of the beta-adrenergic pathway.

This article has been cited by other articles:

				J. A. Kanaley, R. Dall, N. Moller, S. C. Nielsen, J. S. Christiansen, M. D. Jensen, and J. O. L. Jorgensen Acute exposure to GH during exercise stimulates the turnover of free fatty acids in GH-deficient men J Appl Physiol, February 1, 2004; 96(2): 747 - 753. [Abstract] [Full Text] [PDF]

				C. J. Pritzlaff, L. Wideman, J. Blumer, M. Jensen, R. D. Abbott, G. A. Gaesser, J. D. Veldhuis, and A. Weltman Catecholamine release, growth hormone secretion, and energy expenditure during exercise vs. recovery in men J Appl Physiol, September 1, 2000; 89(3): 937 - 946. [Abstract] [Full Text] [PDF]

				J. G. Langendonk, A. E. Meinders, J. Burggraaf, M. Frolich, C. A. M. Roelen, R. C. Schoemaker, A. F. Cohen, and H. Pijl Influence of obesity and body fat distribution on growth hormone kinetics in humans Am J Physiol Endocrinol Metab, November 1, 1999; 277(5): E824 - E829. [Abstract] [Full Text] [PDF]

				T. J. Lauterio, A. Barkan, M. DeAngelo, R. DeMott-Friberg, and R. Ramirez Plasma growth hormone secretion is impaired in obesity-prone rats before onset of diet-induced obesity Am J Physiol Endocrinol Metab, July 1, 1998; 275(1): E6 - E11. [Abstract] [Full Text] [PDF]

				T. D. ETHERTON and D. E. BAUMAN Biology of Somatotropin in Growth and Lactation of Domestic Animals Physiol Rev, July 1, 1998; 78(3): 745 - 761. [Abstract] [Full Text] [PDF]

				A. Cittadini, H. Stromer, S. E. Katz, R. Clark, A. C. Moses, J. P. Morgan, and P. S. Douglas Differential Cardiac Effects of Growth Hormone and Insulin-like Growth Factor1 in the Rat : A Combined In Vivo and In Vitro Evaluation Circulation, February 15, 1996; 93(4): 800 - 809. [Abstract] [Full Text]