AJP - Endo Information on EB 2010
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 262: E627-E630, 1992;
0193-1849/92 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peters, B. J.
Right arrow Articles by Rillema, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peters, B. J.
Right arrow Articles by Rillema, J. A.

AJP - Endocrinology and Metabolism, Vol 262, Issue 5 E627-E630, Copyright © 1992 by American Physiological Society

ARTICLES

Effect of prolactin on 2-deoxyglucose uptake in mouse mammary gland explants

B. J. Peters and J. A. Rillema
Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201.

These studies were carried out to explore the possible effect of prolactin (PRL) on glucose uptake into culture mammary gland explants derived from 12- to 14-day pregnant mice. PRL was found to stimulate an increased rate of uptake of a nonmetabolized glucose analogue, 2-[3H]deoxyglucose, into cultured mammary tissues. The onset of this response was 16 h after the addition of PRL, and the response persisted for at least 24 h. A similar temporal response was observed when the PRL stimulation of [14C]glucose oxidation to 14CO2 was determined. The lowest PRL concentration that elicited a stimulation of 2-deoxyglucose uptake was 20 ng/ml, and a maximum response occurred with PRL at a concentration of 250 ng/ml. Ongoing protein synthesis appears to be essential for PRL to express its effect on 2-deoxyglucose transport since cyclohexamide, puromycin, and actinomycin D abolished the PRL response. It is also apparent that the PRL stimulation of 2-deoxyglucose involves activation of a specific carrier-mediated uptake transport system, since the rate of uptake of L-glucose into mouse mammary gland explants was unaffected by PRL.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. A. Rillema
Effect of prolactin on phosphate transport and incorporation in mouse mammary gland explants
Am J Physiol Endocrinol Metab, July 1, 2002; 283(1): E132 - E137.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
D. B. Shennan and M. Peaker
Transport of Milk Constituents by the Mammary Gland
Physiol Rev, July 1, 2000; 80(3): 925 - 951.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
J. A. Rillema, S. Collins, and C. H. Williams
Prolactin Stimulation of Iodide Uptake and Incorporation into Protein Is Polyamine-Dependent in Mouse Mammary Gland Explants
Experimental Biology and Medicine, May 1, 2000; 224(1): 41 - 44.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online