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Am J Physiol Endocrinol Metab 261: E661-E664, 1991;
0193-1849/91 $5.00
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AJP - Endocrinology and Metabolism, Vol 261, Issue 5 E661-E664, Copyright © 1991 by American Physiological Society

ARTICLES

Calibration method for small animal indirect calorimeters

S. J. MacKay, A. Loiseau, R. Poivre and A. Huot
Institut National de la Sante et de la Recherche Medicale (INSERM) Unite 1, Hopital Bichat, Paris, France.

A simple mini-butane burner or "artificial rat," for calibration of small animal indirect calorimeters (modified Benedict and Haldane systems), was developed. Substitution of this burner for a live animal at regular intervals in an experimental protocol provides a means by which whole system function can be verified without disturbing respirometer conditions. Heat production, degree of total combustion of butane, proportion of unburned butane, and the CO2 production (VCO2)-to-O2 consumption (VO2) ratio were determined in animal and burner experiments by direct measurement or were derived from mass equations. Burner security was also discussed. VCO2/VO2 obtained in calibration experiments using the Benedict system remained constant and was not significantly different from the expected value for total combustion of butane. Observation of a stabilization phase in the Haldane burner experiments illustrated the utility of calibration over the duration of a normal animal experiment. This miniburner gave highly reproducible results and simulated the daily VO2, VCO2, and heat production of an adult rat.


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