AJP - Endo Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 260: E846-E851, 1991;
0193-1849/91 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Boni-Schnetzler, M.
Right arrow Articles by Froesch, E. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Boni-Schnetzler, M.
Right arrow Articles by Froesch, E. R.

AJP - Endocrinology and Metabolism, Vol 260, Issue 6 E846-E851, Copyright © 1991 by American Physiological Society

ARTICLES

Insulin regulates insulin-like growth factor I mRNA in rat hepatocytes

M. Boni-Schnetzler, C. Schmid, P. J. Meier and E. R. Froesch
Metabolic Unit, University Hospital, Zurich, Switzerland.

To evaluate the regulatory role of growth hormone (GH) and insulin on insulin-like growth factor I (IGF-I) mRNA levels, we employed primary rat hepatocytes. Cells were incubated for 16 h with 10 nM insulin, 10 nM GH, or a combination thereof, and IGF-I mRNA levels were analyzed by Northern blotting. Insulin results in 2.5-fold and GH in 3.8-fold higher IGF-I mRNA levels than hormone-free controls, and a combination of insulin and GH had an additive effect (6.7-fold). The effect of 10 nM insulin was constant at variable GH concentrations. Therefore, GH and insulin affect IGF-I mRNA levels independently of each other. The half-maximal effective dose of insulin was 4.7 X 10(-10) M, and, in kinetic experiments, insulin was effective within 2 h. These findings demonstrate that insulin modulates hepatic IGF-I production by a direct regulation of the transcript levels of IGF-I.


This article has been cited by other articles:


Home page
 Eur J EndocrinolHome page
U. Espelund, J. M. Bruun, B. Richelsen, A. Flyvbjerg, and J. Frystyk
Pro- and mature IGF-II during diet-induced weight loss in obese subjects
Eur. J. Endocrinol., December 1, 2005; 153(6): 861 - 869.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Goya, A. de la Puente, S. Ramos, M. A. Martin, F. Escriva, and A. M. Pascual-Leone
Regulation of Insulin-like Growth Factor-I and -II by Glucose in Primary Cultures of Fetal Rat Hepatocytes
J. Biol. Chem., August 27, 1999; 274(35): 24633 - 24640.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. R. Christ, P. V. Carroll, E. Albany, A. M. Umpleby, P. J. Lumb, A. S. Wierzbicki, P. H. Sonksen, and D. L. Russell-Jones
Effect of IGF-I therapy on VLDL apolipoprotein B100 metabolism in type 1 diabetes mellitus
Am J Physiol Endocrinol Metab, May 1, 2002; 282(5): E1154 - E1162.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online