| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Endocrinology and Metabolism, Vol 259, Issue 2 E185-E194, Copyright © 1990 by American Physiological Society
ARTICLES |
W. M. Bennet, A. A. Connacher, C. M. Scrimgeour, R. T. Jung and M. J. Rennie
Department of Anatomy, University of Dundee, United Kingdom.
The effect of insulin on leg and whole body protein turnover was determined by leg exchange and plasma kinetics of [15N]phenylalanine and [1-13C]leucine during amino acid (AA) sufficiency. Eight healthy subjects were studied during AA infusion alone and during infusion of glucose and insulin (0.29 nmol.m-2.min-1) with additional AA. Insulin strongly stimulated the positive leg AA balance seen with AA (AA alone, 2.6 +/- 6.1 vs. insulin + AA, 33.1 +/- 5.8 nmol phenylalanine . 100 g leg-1.min-1; P less than 0.001). Phenylalanine uptake by leg tissues rose during insulin plus AA (47.3 +/- 11.5 vs. 73.1 +/- 7.3 nmol. 100 g-1.min-1; P = 0.022) but with only a slight reduction in leg phenylalanine release (44.7 +/- 8.1 vs. 40.0 +/- 7.9 nmol.100 g-1.min-1). Leg nonoxidative leucine plus alpha-ketoisocaproate (KIC) uptake was increased slightly with insulin (129 +/- 26 vs. 146 +/- 21 nmol.100 g-1. min-1), but leg leucine oxidation increased fourfold (P = 0.012). Leg leucine plus KIC release was reduced by insulin (120 +/- 17 vs. 84 +/- 10 nmol.100 g-1.min-1; P = 0.005); endogenous leucine appearance of leucine and phenylalanine decreased with insulin (leucine, 1.97 +/- 0.08 vs. 1.65 +/- 0.10; phenylalanine, 0.76 +/- 0.03 vs. 0.54 +/- 0.08 mumols.kg-1.min-1; P less than 0.02). The results suggest that insulin, given with sufficient amino acids, may stimulate leg and whole body protein balance by mechanisms including stimulation of protein synthesis and inhibition of protein breakdown.
This article has been cited by other articles:
|
|
 |
|
  P. L. Greenhaff, L. G. Karagounis, N. Peirce, E. J. Simpson, M. Hazell, R. Layfield, H. Wackerhage, K. Smith, P. Atherton, A. Selby, et al. Disassociation between the effects of amino acids and insulin on signaling, ubiquitin ligases, and protein turnover in human muscle Am J Physiol Endocrinol Metab, September 1, 2008; 295(3): E595 - E604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. I. Smith, D. T. Villareal, and B. Mittendorfer Measurement of human mixed muscle protein fractional synthesis rate depends on the choice of amino acid tracer Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E666 - E671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. D. Brown and W. W. Hay Jr. Effect of hyperinsulinemia on amino acid utilization and oxidation independent of glucose metabolism in the ovine fetus Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1333 - E1340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Chow, R. C. Albright, M. L. Bigelow, G. Toffolo, C. Cobelli, and K. S. Nair Mechanism of insulin's anabolic effect on muscle: measurements of muscle protein synthesis and breakdown using aminoacyl-tRNA and other surrogate measures Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E729 - E736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fujita, B. B. Rasmussen, J. G. Cadenas, J. J. Grady, and E. Volpi Effect of insulin on human skeletal muscle protein synthesis is modulated by insulin-induced changes in muscle blood flow and amino acid availability Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E745 - E754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Bell, S. Fujita, E. Volpi, J. G. Cadenas, and B. B. Rasmussen Short-term insulin and nutritional energy provision do not stimulate muscle protein synthesis if blood amino acid availability decreases Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E999 - E1006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Highstead, K. D. Tipton, D. L. Creson, R. R. Wolfe, and A. A. Ferrando Incidence of associated events during the performance of invasive procedures in healthy human volunteers J Appl Physiol, April 1, 2005; 98(4): 1202 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu, G. Li, S. R. Kimball, L. A. Jahn, and E. J. Barrett Glucocorticoids modulate amino acid-induced translation initiation in human skeletal muscle Am J Physiol Endocrinol Metab, August 1, 2004; 287(2): E275 - E281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Paddon-Jones, M. Sheffield-Moore, X.-J. Zhang, E. Volpi, S. E. Wolf, A. Aarsland, A. A. Ferrando, and R. R. Wolfe Amino acid ingestion improves muscle protein synthesis in the young and elderly Am J Physiol Endocrinol Metab, March 1, 2004; 286(3): E321 - E328. [Abstract] [Full Text]
|
|
|
|
|
|
M. Louis, J. R. Poortmans, M. Francaux, J. Berre, N. Boisseau, E. Brassine, D. J. R. Cuthbertson, K. Smith, J. A. Babraj, T. Waddell, et al. No effect of creatine supplementation on human myofibrillar and sarcoplasmic protein synthesis after resistance exercise Am J Physiol Endocrinol Metab, November 1, 2003; 285(5): E1089 - E1094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M J Rennie Claims for the anabolic effects of growth hormone: a case of the Emperor's new clothes? Br. J. Sports Med., April 1, 2003; 37(2): 100 - 105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Louis, J. R. Poortmans, M. Francaux, E. Hultman, J. Berre, N. Boisseau, V. R. Young, K. Smith, W. Meier-Augenstein, J. A. Babraj, et al. Creatine supplementation has no effect on human muscle protein turnover at rest in the postabsorptive or fed states Am J Physiol Endocrinol Metab, April 1, 2003; 284(4): E764 - E770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fouillet, C. Bos, C. Gaudichon, and D. Tome Approaches to Quantifying Protein Metabolism in Response to Nutrient Ingestion J. Nutr., October 1, 2002; 132(10): 3208S - 3218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Rennie, J. Bohe, and R. R. Wolfe Latency, Duration and Dose Response Relationships of Amino Acid Effects on Human Muscle Protein Synthesis J. Nutr., October 1, 2002; 132(10): 3225S - 3227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. L. Calbet and D. A. MacLean Plasma Glucagon and Insulin Responses Depend on the Rate of Appearance of Amino Acids after Ingestion of Different Protein Solutions in Humans J. Nutr., August 1, 2002; 132(8): 2174 - 2182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Caso, G. C. Ford, K. S. Nair, J. A. Vosswinkel, P. J. Garlick, and M. A. McNurlan Increased concentration of tracee affects estimates of muscle protein synthesis Am J Physiol Endocrinol Metab, June 1, 2001; 280(6): E937 - E946. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. G. Whittaker, C. H. Lee, and R. Taylor Whole body protein kinetics in women: effect of pregnancy and IDDM during anabolic stimulation Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E978 - E988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sheffield-Moore, R. R. Wolfe, D. C. Gore, S. E. Wolf, D. M. Ferrer, and A. A. Ferrando Combined effects of hyperaminoacidemia and oxandrolone on skeletal muscle protein synthesis Am J Physiol Endocrinol Metab, February 1, 2000; 278(2): E273 - E279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Leyva, M Rauchhaus, S.D Anker, A.J Proudler, I.F Godsland, P Stiefel, A.J.S Coats, P.A Poole-Wilson, and J.C Stevenson Non-invasive assessment of vascular function. Paradoxical vascular response to intravenous glucose in coronary heart disease Eur. Heart J., January 1, 2000; 21(1): 39 - 44. [Abstract] [PDF]
|
|
|
|
|
|
K. D. Tipton, A. A. Ferrando, S. M. Phillips, D. Doyle Jr., and R. R. Wolfe Postexercise net protein synthesis in human muscle from orally administered amino acids Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E628 - E634. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sinaud, M. Balage, G. Bayle, D. Dardevet, T. C. Vary, S. R. Kimball, L. S. Jefferson, and J. Grizard Diazoxide-induced insulin deficiency greatly reduced muscle protein synthesis in rats: involvement of eIF4E Am J Physiol Endocrinol Metab, January 1, 1999; 276(1): E50 - E61. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Fischman, Y. M. Yu, E. Livni, J. W. Babich, V. R. Young, N. M. Alpert, and R. G. Tompkins Muscle protein synthesis by positron-emission tomography with L-[methyl-11C]methionine in adult humans PNAS, October 27, 1998; 95(22): 12793 - 12798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Wray-Cahen, H. V. Nguyen, D. G. Burrin, P. R. Beckett, M. L. Fiorotto, P. J. Reeds, T. J. Wester, and T. A. Davis Response of skeletal muscle protein synthesis to insulin in suckling pigs decreases with development Am J Physiol Endocrinol Metab, October 1, 1998; 275(4): E602 - E609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Laurichesse, I. Tauveron, F. Gourdon, L. Cormerais,, C. Champredon, S. Charrier, C. Rochon, S. Lamain,, G. Bayle, H. Laveran, et al. Threonine and Methionine Are Limiting Amino Acids for Protein Synthesis in Patients with AIDS J. Nutr., August 1, 1998; 128(8): 1342 - 1348. [Abstract] [Full Text]
|
|
|
|
|
|
T. A. Hillier, D. A. Fryburg, L. A. Jahn, and E. J. Barrett Extreme hyperinsulinemia unmasks insulin's effect to stimulate protein synthesis in the human forearm Am J Physiol Endocrinol Metab, June 1, 1998; 274(6): E1067 - E1074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Roy and M. A. Tarnopolsky Influence of differing macronutrient intakes on muscle glycogen resynthesis after resistance exercise J Appl Physiol, March 1, 1998; 84(3): 890 - 896. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Roy, M. A. Tarnopolsky, J. D. Macdougall, J. Fowles, and K. E. Yarasheski Effect of glucose supplement timing on protein metabolism after resistance training J Appl Physiol, June 1, 1997; 82(6): 1882 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Ferrando, K. D. Tipton, M. M. Bamman, and R. R. Wolfe Resistance exercise maintains skeletal muscle protein synthesis during bed rest J Appl Physiol, March 1, 1997; 82(3): 807 - 810. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
