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Am J Physiol Endocrinol Metab 257: E797-E803, 1989;
0193-1849/89 $5.00
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AJP - Endocrinology and Metabolism, Vol 257, Issue 6 E797-E803, Copyright © 1989 by American Physiological Society

ARTICLES

Endothelin-1 activates phospholipase C and mobilizes Ca2+ from extra- and intracellular pools in osteoblastic cells

Y. Takuwa, Y. Ohue, N. Takuwa and K. Yamashita
Department of Internal Medicine, University of Tsukuba, Ibaraki, Japan.

The effect of endothelin-1 (ET), a novel vasoactive peptide derived from endothelial cells, on osteoblastic MC3T3-E1 cells was studied. ET specifically binds to a single class of high-affinity receptors in MC3T3-E1 cells and induces phospholipase C activation with the production of two second messengers, inositol trisphosphate and 1,2-diacylglycerol, and a biphasic increase in intracellular free Ca2+ concentration ([Ca2+]i), which consists of an initial transient increase and an ensuing sustained plateau, as measured with a fluorescent indicator, fura-2. The second plateau phase but not the initial transient increase in [Ca2+]i induced by ET is abolished by removal of extracellular Ca2+ but not by either nicardipine, verapamil, or diltiazem. The ET-stimulated production of inositol trisphosphate is not abolished by removal of extracellular Ca2+, indicating that ET-stimulated phospholipase C activation is not a consequence of an increase in Ca2+ influx across the plasma membrane. ET causes stimulation of DNA synthesis and reduction of alkaline phosphatase activity in MC3T3-E1 cells. A protein kinase C activator phorbol 12,13-dibutyrate mimics these effects of ET. The results demonstrate that ET activates the inositol lipid signaling pathway and induces mobilization of Ca2+ from both extra- and intracellular pools and activation of protein kinase C in osteoblastic MC3T3-E1 cells.


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 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y. Hiruma, A. Inoue, A. Shiohama, E. Otsuka, S. Hirose, A. Yamaguchi, and H. Hagiwara
Endothelins inhibit the mineralization of osteoblastic MC3T3-E1 cells through the A-type endothelin receptor
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 1998; 275(4): R1099 - R1105.
[Abstract] [Full Text] [PDF]


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