AJP - Endocrinology and Metabolism, Vol 257, Issue 2 E235-E240, Copyright © 1989 by American Physiological Society
[Ala6]gastrin-releasing peptide-10: an analogue with dissociated biological activities
H. Mukai, K. Kawai, S. Suzuki, H. Ohmori, K. Yamashita and E. Munekata
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
COOH-terminal decapeptide of gastrin-releasing peptide (GRP-10) is a
bombesin-like peptide, which has bioactivities to stimulate gastrin,
insulin, and glucagon secretion. We have synthesized an analogue of GRP-10
that inhibits GRP-10's stimulation of insulin secretion both in vivo and in
vitro and glucagon secretion in vivo, while potentiating the stimulation of
gastrin secretion. The amino acid sequence of this peptide is
H-Gly-Asn-Trp-Ala-Ala-Gly-His-Leu-Met-NH2 ([Ala6]GRP-10). Because the
stimulation of insulin and gastrin secretion by GRP-10 has been ascribed to
a direct effect on B- and G-cells, these findings suggest that there are
two subtypes of receptors for bombesin-like peptides in mammalian tissues.
