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Am J Physiol Endocrinol Metab 256: E550-E558, 1989;
0193-1849/89 $5.00
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AJP - Endocrinology and Metabolism, Vol 256, Issue 4 E550-E558, Copyright © 1989 by American Physiological Society

ARTICLES

Transport of glutamine across blood-facing membranes of perfused rat jejunum

P. M. Taylor, C. J. Egan and M. J. Rennie
Department of Anatomy and Physiology, University of Dundee, United Kingdom.

Transport of glutamine and other neutral amino acids across the blood-facing membranes of isolated, dually perfused rat jejunum was measured using a paired-tracer isotope-dilution technique. Glutamine, asparagine, histidine, alanine, and leucine showed mutual inhibition of transport. The major component of physiological glutamine transport was saturable (Km = 0.88 +/- 0.15 mM, Vmax = 454 +/- 49 nmol.g-1.min-1; mean +/- SE), stereospecific and Na-independent and appeared to exhibit symmetry of glutamine transport; it most resembled system L. The minor Na-dependent component of glutamine transport resembled system A, i.e., it transported N-methylaminoisobutyric acid (Km approximately equal to 10 microM, Vmax approximately equal to 1.2 nmol.g-1.min-1). At 0.5 mM glutamine transport was insensitive to insulin and glucagon and was unaffected by perfusate pH (7.0-7.8). Glutamine extracted by the jejunum is rapidly utilized; at physiological blood glutamine concentrations the basolateral glutamine-transporter flux may thus not only restrict intestinal glutamine catabolism but also the consequent release of glutamine-derived ammonia (a substrate and stimulant of ureogenesis) into the portal circulation.


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