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Am J Physiol Endocrinol Metab 254: E562-E565, 1988;
0193-1849/88 $5.00
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AJP - Endocrinology and Metabolism, Vol 254, Issue 5 E562-E565, Copyright © 1988 by American Physiological Society

ARTICLES

Choice of infusion-sampling mode for tracer studies of free fatty acid metabolism

M. D. Jensen, P. J. Rogers, M. G. Ellman and J. M. Miles
Department of Internal Medicine, Mayo Medical School, Rochester, Minnesota 55905.

To determine the preferred infusion-sampling mode for isotopic studies of free fatty acid (FFA) metabolism, tracer [( 14C]palmitate) was infused into the left ventricle of five anesthetized dogs, and tracee ([3H]palmitate) was infused into three separate peripheral veins of each dog. The [14C]palmitate specific activity (SA) was lower in mixed venous than arterial blood, and [3H]palmitate SA was equal in both sites. The actual infusion rate of [3H]palmitate [2.15 +/- 0.31 X 10(5) disintegrations/min (dpm).kg-1.min-1] could be accurately predicted (2.14 +/- 0.32 X 10(5) dpm.kg-1.min-1) using the known [14C]palmitate infusion rate and the arterial plasma [14C]-to-[3H]palmitate ratio. In contrast, the mixed venous [14C]-to-[3H]palmitate ratio resulted in overestimates (P less than 0.05) of the actual [3H]palmitate infusion rate. In summary, venous tracer infusion with arterial blood sampling for FFA tracer studies provides the most accurate estimates of tracee rate of appearance.


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