| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Endocrinology and Metabolism, Vol 254, Issue 2 E208-E213, Copyright © 1988 by American Physiological Society
ARTICLES |
K. S. Nair, D. Halliday and R. C. Griggs
Department of Medicine, University of Rochester School of Medicine and Dentistry, New York 14603-0905.
Fractional mixed skeletal muscle protein synthesis (FMPS) was estimated in 10 postabsorptive healthy men by determining the increment in the abundance of [13C]-leucine in quadriceps muscle protein during an intravenous infusion of L-[1-13C]leucine. FMPS in our subjects was 0.046 +/- 0.003%/h. Whole-body muscle protein synthesis (MPS) was calculated based on the estimation of muscle mass from creatinine excretion and compared with whole-body protein synthesis (WBPS) calculated from the nonoxidative portion of leucine flux. A significant correlation (r2 = 0.73, P less than 0.05) was found between MPS (44.7 +/- 3.4 mg.kg-1.h-1) and WBPS (167.8 +/- 8.5 mg.kg-1.h-1). The contribution of MPS to WBPS was 27 +/- 1%, which is comparable to the reports in other species. Morphometric analyses of adjacent muscle samples in eight subjects demonstrated that the biopsy specimens consisted of 86.5 +/- 2% muscular as opposed to other tissues. Because fiber type composition varies between biopsies, we examined the relationship between proportions of each fiber type and FMPS. Variation in the composition of biopsies and in fiber-type proportion did not affect the estimation of muscle protein synthesis rate. We conclude that stable isotope techniques using serial needle biopsies permit the direct measurement of FMPS in humans and that this estimation is correlated with an indirect estimation of WBPS.
This article has been cited by other articles:
|
|
 |
|
  A. Jaleel, V. Nehra, X.-M. T. Persson, Y. Boirie, M. Bigelow, and K. S. Nair In vivo measurement of synthesis rate of multiple plasma proteins in humans Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E190 - E197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Balagopal, R. Olney, D. Darmaun, E. Mougey, M. Dokler, G. Sieck, and D. Hammond Oxandrolone enhances skeletal muscle myosin synthesis and alters global gene expression profile in Duchenne muscular dystrophy Am J Physiol Endocrinol Metab, March 1, 2006; 290(3): E530 - E539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. S. Nair, A. Jaleel, Y. W. Asmann, K. R. Short, and S. Raghavakaimal Proteomic research: potential opportunities for clinical and physiological investigators Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E863 - E874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. R. Short, J. L. Vittone, M. L. Bigelow, D. N. Proctor, and K. S. Nair Age and aerobic exercise training effects on whole body and muscle protein metabolism Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E92 - E101. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Xu, Z. Fei, Y.-M. Yu, W. Xu, A. Rhodes, R. G. Tompkins, and J. T. Schulz In vivo rabbit hindquarter model for assessment of regional burn hypermetabolism J Appl Physiol, January 1, 2003; 94(1): 135 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Liu and E. J. Barrett Human protein metabolism: its measurement and regulation Am J Physiol Endocrinol Metab, December 1, 2002; 283(6): E1105 - E1112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-J. Zhang, D. L. Chinkes, and R. R. Wolfe Measurement of muscle protein fractional synthesis and breakdown rates from a pulse tracer injection Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E753 - E764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Halvatsiotis, K. R. Short, M. Bigelow, and K. S. Nair Synthesis Rate of Muscle Proteins, Muscle Functions, and Amino Acid Kinetics in Type 2 Diabetes Diabetes, August 1, 2002; 51(8): 2395 - 2404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sreekumar, P. Halvatsiotis, J. C. Schimke, and K. S. Nair Gene Expression Profile in Skeletal Muscle of Type 2 Diabetes and the Effect of Insulin Treatment Diabetes, June 1, 2002; 51(6): 1913 - 1920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Balagopal, J. C. Schimke, P. Ades, D. Adey, and K. S. Nair Age effect on transcript levels and synthesis rate of muscle MHC and response to resistance exercise Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E203 - E208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Hasten, J. Pak-Loduca, K. A. Obert, and K. E. Yarasheski Resistance exercise acutely increases MHC and mixed muscle protein synthesis rates in 78-84 and 23-32 yr olds Am J Physiol Endocrinol Metab, April 1, 2000; 278(4): E620 - E626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Adey, R. Kumar, J. T. McCarthy, and K. S. Nair Reduced synthesis of muscle proteins in chronic renal failure Am J Physiol Endocrinol Metab, February 1, 2000; 278(2): E219 - E225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Yarasheski, J. Pak-Loduca, D. L. Hasten, K. A. Obert, M. B. Brown, and D. R. Sinacore Resistance exercise training increases mixed muscle protein synthesis rate in frail women and men >= 76 yr old Am J Physiol Endocrinol Metab, July 1, 1999; 277(1): E118 - E125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease . A Statement of the American Thoracic Society and European Respiratory Society Am. J. Respir. Crit. Care Med., April 1, 1999; 159(4): S2 - 40. [Full Text] [PDF]
|
|
|
|
|
|
A. Fu and K. S. Nair Age effect on fibrinogen and albumin synthesis in humans Am J Physiol Endocrinol Metab, December 1, 1998; 275(6): E1023 - E1030. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Hasten, G. S. Morris, S. Ramanadham, and K. E. Yarasheski Isolation of human skeletal muscle myosin heavy chain and actin for measurement of fractional synthesis rates Am J Physiol Endocrinol Metab, December 1, 1998; 275(6): E1092 - E1099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Yarasheski, J. J. Zachwieja, J. Gischler, J. Crowley, M. M. Horgan, and W. G. Powderly Increased plasma Gln and Leu Ra and inappropriately low muscle protein synthesis rate in AIDS wasting Am J Physiol Endocrinol Metab, October 1, 1998; 275(4): E577 - E583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Balagopal, O. E. Rooyackers, D. B. Adey, P. A. Ades, and K. S. Nair Effects of aging on in vivo synthesis of skeletal muscle myosin heavy-chain and sarcoplasmic protein in humans Am J Physiol Endocrinol Metab, October 1, 1997; 273(4): E790 - E800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Roy, M. A. Tarnopolsky, J. D. Macdougall, J. Fowles, and K. E. Yarasheski Effect of glucose supplement timing on protein metabolism after resistance training J Appl Physiol, June 1, 1997; 82(6): 1882 - 1888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. E. Rooyackers, D. B. Adey, P. A. Ades, and K. S. Nair Effect of age on in vivo rates of mitochondrial protein synthesis in human skeletal muscle PNAS, December 24, 1996; 93(26): 15364 - 15369. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
