AJP - Endocrinology and Metabolism, Vol 253, Issue 2 E130-E134, Copyright © 1987 by American Physiological Society
IP3-dependent Ca2+ release in permeabilized hepatocytes of endotoxemic and septic rats
J. A. Spitzer and I. V. Deaciuc
Inositol trisphosphate-dependent Ca2+ release was measured in
saponin-permeabilized hepatocytes isolated from acutely (2 mg/100 g body wt
iv) or chronically (0.1 mg X 100 g body wt-1 X 24 h-1 for 30 h)
endotoxin-treated (ET, Escherichia coli) rats or from animals rendered
septic by cecal ligation and puncture. A decrease of this parameter was
observed in acutely ET-treated rats (52%, P less than 0.01) and after 30 h
of continuous ET infusion (33%, P less than 0.01). Sepsis was associated
with an elevated Ca2+ release (34%, P less than 0.01) as compared with the
sham-operated animals. We conclude that during endotoxicosis and sepsis
alterations of intracellular Ca homeostasis take place, reaching sites
beyond the level of the plasma membrane. Such alterations could account in
part for metabolic and functional changes associated with these pathologic
states. In addition, ET treatment provides the first known intervention
resulting in the modulation of inositol trisphosphate-dependent Ca2+
release.
