AJP - Endocrinology and Metabolism, Vol 252, Issue 6 E778-E782, Copyright © 1987 by American Physiological Society
Metabolic degradation and synthesis of calcitriol in spontaneously hypertensive rat
E. W. Young, C. H. Hsu, S. Patel, R. U. Simpson and P. Komanicky
Plasma calcitriol concentration is unexpectedly low in spontaneously
hypertensive rats (SHR) compared with normotensive Wistar-Kyoto rats (WKY)
in view of previous findings of low plasma ionized calcium,
hypophosphatemia, and hyperparathyroidism in SHR. Also, parathyroid hormone
(PTH) and other exogenous stimuli cause a smaller increment in plasma
calcitriol in SHR than in WKY. We investigated whether these differences in
plasma calcitriol levels were due to decreased production rate (PR) or
increased metabolic clearance rate (MCR) in SHR using the continuous
infusion-isotope equilibration technique. In the basal state, MCR was 0.306
+/- 0.048 ml X min-1 X kg-1 in SHR and 0.284 +/- 0.026 ml X min-1 X kg-1 in
WKY (P = NS). Estimated PR was lower in SHR (22.7 vs. 33.7 ng X day-1 X
kg-1). With PTH infusion (3 U X h-1 X 100 g-1 for 20 h), MCR was 0.220 +/-
0.011 ml X min-1 X kg-1 in SHR and 0.256 +/- 0.017 ml X min-1 X kg-1 in WKY
(P = NS). Measured PR was markedly lower in SHR (154 +/- 24 vs. 262 +/- 27
ng X day-1 X kg-1; P less than 0.02). Basal plasma PTH, total plasma
calcium, and plasma inorganic phosphate were not different between SHR and
WKY. Thus, SHR show diminished calcitriol production under basal condition
and with PTH stimulation. MCR is not increased in SHR and does not appear
to be influenced by PTH or the accompanying elevation in plasma calcium and
calcitriol.
