AJP - Endocrinology and Metabolism, Vol 251, Issue 5 597-E603, Copyright © 1986 by American Physiological Society
Hormonal responses associated with early hyperglycemia after graded hemorrhage in dogs
M. K. McLeod, D. E. Carlson and D. S. Gann
Trained, awake, splenectomized dogs (n = 17) were studied to examine
potential mechanisms for early hyperglycemia after hemorrhage (H). Animals
were surgically prepared under halothane-nitrous oxide 3 days before the
experiment. Chronic catheters were placed aseptically in the portal vein
(PV), femoral vein, and femoral artery. Electromagnetic flow probes were
placed around the PV and hepatic artery. After an overnight fast, dogs were
hemorrhaged 10, 20, or 30% of their estimated blood volume in 3 min. Flow
measurements and blood samples for glucose (G), immunoreactive insulin
(IRI), immunoreactive glucagon (IRG), catecholamines (C), and cortisol (F)
were taken prior to H and from 5 min to 8 h post-H. Plasma IRI, IRG, and F
were measured by radioimmunoassay, plasma C by high-pressure liquid
chromatography, and G by a glucose oxidase method. Peripheral G did not
change after 10% H but increased significantly after 20 and 30% H from 10
min to 2 h. Similarly, peripheral C did not change after 10% H but
increased significantly from 10 min to 2 h after 20 and 30% H. In contrast,
the portal venous delivery of IRG did not increase significantly until at
least 1 h after any magnitude of H. Peripheral IRI did not change after any
magnitude of H. However, portal venous delivery of IRI decreased
significantly from 20 min to 6 h after 10% H. Plasma F increased
significantly in peripheral blood after all magnitudes of H. These results
indicate that an increase in the release of IRG occurs too late to account
for the early hyperglycemia that occurs during the 1st h following
H.(ABSTRACT TRUNCATED AT 250 WORDS)
