| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AJP - Endocrinology and Metabolism, Vol 251, Issue 1 117-E126, Copyright © 1986 by American Physiological Society
ARTICLES |
D. Darmaun, D. E. Matthews and D. M. Bier
To study glutamate and glutamine kinetics, 4-h unprimed intravenous infusions of L-[15N]glutamate, L-[2-15N]glutamine, and L-[5-15N]-glutamine were administered to healthy young adult male subjects in the postabsorptive state. Arterialized-venous blood samples were drawn and analyzed for glutamate and glutamine 15N enrichments. The fractional turnover rates of the tracer-miscible glutamate and glutamine pools were fast, 8.0 and 2.8% min-1, respectively. The glutamate tracer-miscible pool accounted for less than one-tenth the estimated free glutamate pool in the body. The plasma glutamate amino N, glutamine amino N and glutamine amide N rates of appearance were 83 +/- 22 (means +/- SD), 348 +/- 33, and 283 +/- 31 mumol X kg-1 X h-1, respectively. The glutamine amide N appearance rate was 20% slower than the amino N appearance rate, indicating that glutamine transaminase is an active pathway in human glutamine metabolism. From measurement of transfer of tracer 15N, we found that only 5% of the glutamine synthesized in cells and released into plasma was derived from intracellular glutamate that had mixed with plasma. These data demonstrate that intravenously administered tracers of glutamate or glutamine do not mix thoroughly with the intracellular pools, and their measured kinetics reflect transport rates through plasma rather than whole-body fluxes.
This article has been cited by other articles:
|
|
 |
|
  F. Agostini, M. Heer, G. Guarnieri, and G. Biolo Physical inactivity decreases whole body glutamine turnover independently from changes in proteolysis J. Physiol., October 1, 2008; 586(19): 4775 - 4781. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Thibault, S. Welch, N. Mauras, B. Sager, A. Altomare, M. Haymond, and D. Darmaun Corticosteroids increase glutamine utilization in human splanchnic bed Am J Physiol Gastrointest Liver Physiol, February 1, 2008; 294(2): G548 - G553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Phanvijhitsiri, M. W. Musch, M. J. Ropeleski, and E. B. Chang Heat induction of heat shock protein 25 requires cellular glutamine in intestinal epithelial cells Am J Physiol Cell Physiol, August 1, 2006; 291(2): C290 - C299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Yarasheski, S. R. Smith, and W. G. Powderly Reducing plasma HIV RNA improves muscle amino acid metabolism Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E278 - E284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Rakotoambinina, L. Marks, A. M. Badran, F. Igliki, F. Thuillier, P. Crenn, B. Messing, and D. Darmaun Taurine kinetics assessed using [1,2-13C2]taurine in healthy adult humans Am J Physiol Endocrinol Metab, August 1, 2004; 287(2): E255 - E262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Fuchs, J. C. Perez, J. E. Suetterlin, S. B. Chaudhry, and B. P. Bode Inducible antisense RNA targeting amino acid transporter ATB0/ASCT2 elicits apoptosis in human hepatoma cells Am J Physiol Gastrointest Liver Physiol, March 1, 2004; 286(3): G467 - G478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Battezzati, S. Benedini, A. Fattorini, M. Losa, P. Mortini, S. Bertoli, R. Lanzi, G. Testolin, G. Biolo, and L. Luzi Insulin action on protein metabolism in acromegalic patients Am J Physiol Endocrinol Metab, April 1, 2003; 284(4): E823 - E829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Battezzati, A. Caumo, A. Fattorini, L. P. Sereni, J. Coppa, R. Romito, M. Ammatuna, E. Regalia, V. Mazzaferro, and L. Luzi Amino Acid Kinetics During the Anhepatic Phase of Liver Transplantation Diabetes, June 1, 2002; 51(6): 1690 - 1698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Watford Net Interorgan Transport of L-Glutamate in Rats Occurs via the Plasma, Not via Erythrocytes J. Nutr., May 1, 2002; 132(5): 952 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. R. Young and A. M. Ajami Glutamine: The Emperor or His Clothes? J. Nutr., September 1, 2001; 131(9): 2449S - 2459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. P. Bode Recent Molecular Advances in Mammalian Glutamine Transport J. Nutr., September 1, 2001; 131(9): 2475S - 2485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Newsholme Why Is L-Glutamine Metabolism Important to Cells of the Immune System in Health, Postinjury, Surgery or Infection? J. Nutr., September 1, 2001; 131(9): 2515S - 2522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Furst New Developments in Glutamine Delivery J. Nutr., September 1, 2001; 131(9): 2562S - 2568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mittendorfer, E. Volpi, and R. R. Wolfe Whole body and skeletal muscle glutamine metabolism in healthy subjects Am J Physiol Endocrinol Metab, February 1, 2001; 280(2): E323 - E333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Hankard, M. W. Haymond, and D. Darmaun Role of glucose in the regulation of glutamine metabolism in health and in type 1 insulin-dependent diabetes Am J Physiol Endocrinol Metab, September 1, 2000; 279(3): E608 - E613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. H. Forslund, L. Hambraus, H. van Beurden, U. Holmback, A. E. El-Khoury, G. Hjorth, R. Olsson, M. Stridsberg, L. Wide, T. Akerfeldt, et al. Inverse relationship between protein intake and plasma free amino acids in healthy men at physical exercise Am J Physiol Endocrinol Metab, May 1, 2000; 278(5): E857 - E867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. S Kuhn, K. Schuhmann, P. Stehle, D. Darmaun, and P. Furst Determination of glutamine in muscle protein facilitates accurate assessment of proteolysis and de novo synthesis–derived endogenous glutamine production Am. J. Clinical Nutrition, October 1, 1999; 70(4): 484 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Powell-Tuck, C P Jamieson, G E A Bettany, O Obeid, H V Fawcett, C Archer, and D L Murphy A double blind, randomised, controlled trial of glutamine supplementation in parenteral nutrition Gut, July 1, 1999; 45(1): 82 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Lohmann, W. W. Souba, and B. P. Bode Rat liver endothelial cell glutamine transporter and glutaminase expression contrast with parenchymal cells Am J Physiol Gastrointest Liver Physiol, March 1, 1999; 276(3): G743 - G750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. C. Jackson, P. V. Carroll, D. L. Russell-Jones, P. H. Sonksen, D. F. Treacher, and A. M. Umpleby The metabolic consequences of critical illness: acute effects on glutamine and protein metabolism Am J Physiol Endocrinol Metab, January 1, 1999; 276(1): E163 - E170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Yarasheski, J. J. Zachwieja, J. Gischler, J. Crowley, M. M. Horgan, and W. G. Powderly Increased plasma Gln and Leu Ra and inappropriately low muscle protein synthesis rate in AIDS wasting Am J Physiol Endocrinol Metab, October 1, 1998; 275(4): E577 - E583. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Darmaun, S. Welch, A. Rini, B. K. Sager, A. Altomare, and M. W. Haymond Phenylbutyrate-induced glutamine depletion in humans: effect on leucine metabolism Am J Physiol Endocrinol Metab, May 1, 1998; 274(5): E801 - E807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Stumvoll, C. Meyer, G. Perriello, M. Kreider, S. Welle, and J. Gerich Human kidney and liver gluconeogenesis: evidence for organ substrate selectivity Am J Physiol Endocrinol Metab, May 1, 1998; 274(5): E817 - E826. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Visit Other APS Journals Online |
