AJP - Endocrinology and Metabolism, Vol 250, Issue 6 655-E661, Copyright © 1986 by American Physiological Society

ARTICLES

Effects of short-term hyperglycemia on insulin secretion in normal humans

R. E. Ferner, L. Ashworth, B. Tronier and K. G. Alberti

Stepwise glucose clamps were used to study beta-cell insulin response to glucose in normal and noninsulin-dependent diabetic subjects and to study changes in response after hyperglycemia. In normal subjects, insulin increment, delta I, correlated with glucose increment above basal, delta G, during the first 6 min of hyperglycemia, r = 0.748, P less than 0.0001. After 1 h of hyperglycemia, mean delta I/ delta G was reduced from 50 to 23 (mean difference 23 +/- 5) pmol/mmol, P = 0.0002; but delta I/% change in glucose (delta G') was unaltered (2.3 vs. 1.7, mean difference 0.4 +/- 0.3 pmol/%). Second-phase response correlated with mean glucose elevation (r = 0.835, P less than 0.0001), and no plateau was reached after 3 h at 3 mmol/l above basal glucose (rate of change of insulin concentration = 0.5; range, 0.3-0.8 pmol . l-1 . min-1). In diabetic subjects, delta I/ delta G was 20% of normal, while delta I/ delta G' was 63% of normal and second-phase response 30% of normal. We conclude that hyperglycemia per se reduces delta I/ delta G and must be considered when assessing insulin responses. Noninsulin-dependent diabetic subjects have defective first- and second-phase responses.

This article has been cited by other articles:

				E. Toschi, S. Camastra, A. M. Sironi, A. Masoni, A. Gastaldelli, A. Mari, E. Ferrannini, and A. Natali Effect of Acute Hyperglycemia on Insulin Secretion in Humans Diabetes, February 1, 2002; 51(90001): S130 - 133. [Abstract] [Full Text] [PDF]