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Am J Physiol Endocrinol Metab 250: E502-E511, 1986;
0193-1849/86 $5.00
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AJP - Endocrinology and Metabolism, Vol 250, Issue 5 502-E511, Copyright © 1986 by American Physiological Society

ARTICLES

In vitro exhaustion of pancreatic beta-cells

M. Hoenig, L. C. MacGregor and F. M. Matschinsky

To learn more about possible limited beta-cell secretory capacity and factors essential for insulin release, a perifusion system was applied that allowed the in vitro study of insulin secretion from isolated pancreatic islets for more than 6 h. Islets isolated from rats were stimulated with various glucose concentrations (7.5, 16.7, and 30 mM), alpha-ketoisocaproate (30 mM), and 30 mM glucose plus 1 mM 3-isobutyl-1-methylxanthine for several hours in Krebs-Ringer-bicarbonate buffer (KRB) or RPMI 1640. Islets showed "exhaustion" with all stimulatory conditions used when KRB was the perifusion medium. This was not prevented by addition of amino acids, phosphate, myo-inositol or arachidonic acid. With RPMI 1640 as the basal medium, exhaustion was not seen at 7.5 mM but was readily approached at higher glucose concentrations. It is possible that the exhaustion phenomenon observed here is due to a depletion of a readily releasable insulin pool.


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