AJP - Endocrinology and Metabolism, Vol 250, Issue 1 24-E30, Copyright © 1986 by American Physiological Society
Amino acid metabolism after lambda-carrageenan injury to rat skeletal muscle
J. E. Albina, J. Shearer, B. Mastrofrancesco and M. D. Caldwell
To evaluate whether the alterations in amino acid (AA) metabolism that
follow the lambda-carrageenan injury are direct consequences of wounding
and are therefore independent of food intake, plasma AA, in vivo muscle
intracellular free AA, and AA release during isolated hindlimb perfusions
were determined in pentobarbital-anesthetized rats with
lambda-carrageenan-induced hindlimb muscle wounds (W) and in pair-fed (PFC)
and ad libitum-fed (ALC) non-wounded controls. Both PFC and W animals
showed different plasma and muscle intracellular AA composition that ALC.
The alterations observed in these compartments in W animals may not have
resulted exclusively as a consequence of the wound. Wounded hindlimbs
released more AA during perfusion than either control group. The marked
increase in net protein catabolism of W muscle appeared to be related to
wounding and not a consequence of partial starvation. The normalized
release (amino acid/phenylalanine ratio) of glutamine (W less than ALC),
alanine (W less than PFC and ALC), and the branched-chain amino acids (W
greater than PFC and ALC) differed among groups, suggesting an alteration
in the metabolism of these AA in W tissue.
