AJP - Endo Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 248: E353-E362, 1985;
0193-1849/85 $5.00
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kraegen, E. W.
Right arrow Articles by Chisholm, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kraegen, E. W.
Right arrow Articles by Chisholm, D. J.

AJP - Endocrinology and Metabolism, Vol 248, Issue 3 353-E362, Copyright © 1985 by American Physiological Society

ARTICLES

Dose-response curves for in vivo insulin sensitivity in individual tissues in rats

E. W. Kraegen, D. E. James, A. B. Jenkins and D. J. Chisholm

A technique is described for examining in vivo insulin action on glucose utilization in individual tissues in the intact conscious rat. Indices of tissue glucose metabolic rate Rg' and of the percentage of total glucose uptake incorporated into specific storage products (Cf) are derived from tissue analysis after bolus administration of 2-[3H]deoxyglucose and [14C]glucose during the plateau phase of the euglycemic clamp. The effects of insulin elevation have been examined in several tissues. Rg' in diaphragm increased 10-fold over basal (maximal) with a half-maximal sensitivity (ED50) of 150 mU/l. This was similar to the ED50 for net whole body glucose utilization of 133 mU/l. In adipose tissue Rg' increased by twofold and Cf into lipids by sixfold; both were near maximal at 150 mU/l (ED50 of 60 mU/l). A small but significant insulin effect (Rg' increased 2-fold) was found in lung. Insulin did not significantly increase Cf into total liver lipids or glycogen. The methodology described here significantly increases the usefulness of the glucose clamp technique in the study of insulin action. Dose-response curves for insulin action during the euglycemic clamp vary considerably among different target tissues in the rat.


This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. Polkinghorne, Q. Lau, G. J. Cooney, E. W. Kraegen, and M. E. Cleasby
Local activation of the I{kappa}K-NF-{kappa}B pathway in muscle does not cause insulin resistance
Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E316 - E325.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. M. De Taeye, T. Novitskaya, O. P. McGuinness, L. Gleaves, M. Medda, J. W. Covington, and D. E. Vaughan
Macrophage TNF-{alpha} contributes to insulin resistance and hepatic steatosis in diet-induced obesity
Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E713 - E725.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
P. T. Fueger, C. Y. Li, J. E. Ayala, J. Shearer, D. P. Bracy, M. J. Charron, J. N. Rottman, and D. H. Wasserman
Glucose kinetics and exercise tolerance in mice lacking the GLUT4 glucose transporter
J. Physiol., July 15, 2007; 582(2): 801 - 812.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
N. D. Oakes, P. Thalen, E. Aasum, A. Edgley, T. Larsen, S. M. Furler, B. Ljung, and D. Severson
Cardiac metabolism in mice: tracer method developments and in vivo application revealing profound metabolic inflexibility in diabetes
Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E870 - E881.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. A. Maddux, Y.-N. Chang, D. Accili, O. P. McGuinness, J. F. Youngren, and I. D. Goldfine
Overexpression of the insulin receptor inhibitor PC-1/ENPP1 induces insulin resistance and hyperglycemia
Am J Physiol Endocrinol Metab, April 1, 2006; 290(4): E746 - E749.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. Shearer, P. T. Fueger, D. P. Bracy, D. H. Wasserman, and J. N. Rottman
Partial Gene Deletion of Heart-Type Fatty Acid-Binding Protein Limits the Severity of Dietary-Induced Insulin Resistance
Diabetes, November 1, 2005; 54(11): 3133 - 3139.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Shearer, P. T. Fueger, J. N. Rottman, D. P. Bracy, P. H. Martin, and D. H. Wasserman
AMPK stimulation increases LCFA but not glucose clearance in cardiac muscle in vivo
Am J Physiol Endocrinol Metab, November 1, 2004; 287(5): E871 - E877.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. M. Wheatley, S. Rattigan, S. M. Richards, E. J. Barrett, and M. G. Clark
Skeletal muscle contraction stimulates capillary recruitment and glucose uptake in insulin-resistant obese Zucker rats
Am J Physiol Endocrinol Metab, October 1, 2004; 287(4): E804 - E809.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. C. Connolly, L. N. Aglione, M. S. Smith, D. B. Lacy, and M. C. Moore
Insulin action during late pregnancy in the conscious dog
Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E909 - E915.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. C. Sugden and M. J. Holness
Potential Role of Peroxisome Proliferator-Activated Receptor-{alpha} in the Modulation of Glucose-Stimulated Insulin Secretion
Diabetes, February 1, 2004; 53(90001): S71 - 81.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. T. Fueger, D. P. Bracy, C. M. Malabanan, R. R. Pencek, and D. H. Wasserman
Distributed control of glucose uptake by working muscles of conscious mice: roles of transport and phosphorylation
Am J Physiol Endocrinol Metab, January 1, 2004; 286(1): E77 - E84.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
P. T. Fueger, S. Heikkinen, D. P. Bracy, C. M. Malabanan, R. R. Pencek, M. Laakso, and D. H. Wasserman
Hexokinase II partial knockout impairs exercise-stimulated glucose uptake in oxidative muscles of mice
Am J Physiol Endocrinol Metab, November 1, 2003; 285(5): E958 - E963.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. Zhang, C. M. Wheatley, S. M. Richards, E. J. Barrett, M. G. Clark, and S. Rattigan
TNF-{alpha} acutely inhibits vascular effects of physiological but not high insulin or contraction
Am J Physiol Endocrinol Metab, September 1, 2003; 285(3): E654 - E660.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J.-M. Ye, M. A. Iglesias, D. G. Watson, B. Ellis, L. Wood, P. B. Jensen, R. V. Sorensen, P. J. Larsen, G. J. Cooney, K. Wassermann, et al.
PPARalpha /gamma ragaglitazar eliminates fatty liver and enhances insulin action in fat-fed rats in the absence of hepatomegaly
Am J Physiol Endocrinol Metab, March 1, 2003; 284(3): E531 - E540.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. A. Petersen, P. T. Fueger, D. P. Bracy, D. H. Wasserman, and A. E. Halseth
Fiber type-specific determinants of Vmax for insulin-stimulated muscle glucose uptake in vivo
Am J Physiol Endocrinol Metab, March 1, 2003; 284(3): E541 - E548.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. G. Wallis, C. M. Wheatley, S. Rattigan, E. J. Barrett, A. D.H. Clark, and M. G. Clark
Insulin-Mediated Hemodynamic Changes Are Impaired in Muscle of Zucker Obese Rats
Diabetes, December 1, 2002; 51(12): 3492 - 3498.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. A. Iglesias, J.-M. Ye, G. Frangioudakis, A. K. Saha, E. Tomas, N. B. Ruderman, G. J. Cooney, and E. W. Kraegen
AICAR Administration Causes an Apparent Enhancement of Muscle and Liver Insulin Action in Insulin-Resistant High-Fat-Fed Rats
Diabetes, October 1, 2002; 51(10): 2886 - 2894.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
G. W. Cline, K. Johnson, W. Regittnig, P. Perret, E. Tozzo, L. Xiao, C. Damico, and G. I. Shulman
Effects of a Novel Glycogen Synthase Kinase-3 Inhibitor on Insulin-Stimulated Glucose Metabolism in Zucker Diabetic Fatty (fa/fa) Rats
Diabetes, October 1, 2002; 51(10): 2903 - 2910.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. M. Chalkley, M. Hettiarachchi, D. J. Chisholm, and E. W. Kraegen
Long-term high-fat feeding leads to severe insulin resistance but not diabetes in Wistar rats
Am J Physiol Endocrinol Metab, June 1, 2002; 282(6): E1231 - E1238.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
P. W. Hruz, H. Murata, H. Qiu, and M. Mueckler
Indinavir Induces Acute and Reversible Peripheral Insulin Resistance in Rats
Diabetes, April 1, 2002; 51(4): 937 - 942.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
L. H. Clerk, S. Rattigan, and M. G. Clark
Lipid Infusion Impairs Physiologic Insulin-Mediated Capillary Recruitment and Muscle Glucose Uptake In Vivo
Diabetes, April 1, 2002; 51(4): 1138 - 1145.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
P. J. Voshol, M. C. Jong, V. E.H. Dahlmans, D. Kratky, S. Levak-Frank, R. Zechner, J. A. Romijn, and L. M. Havekes
In Muscle-Specific Lipoprotein Lipase-Overexpressing Mice, Muscle Triglyceride Content Is Increased Without Inhibition of Insulin-Stimulated Whole-Body and Muscle-Specific Glucose Uptake
Diabetes, November 1, 2001; 50(11): 2585 - 2590.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. E. Halseth, D. P. Bracy, and D. H. Wasserman
Functional limitations to glucose uptake in muscles comprised of different fiber types
Am J Physiol Endocrinol Metab, June 1, 2001; 280(6): E994 - E999.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
T.-S. TSAO, J. LI, K. S. CHANG, A. E. STENBIT, D. GALUSKA, J. E. ANDERSON, J. R. ZIERATH, R. J. MCCARTER, and M. J. CHARRON
Metabolic adaptations in skeletal muscle overexpressing GLUT4: effects on muscle and physical activity
FASEB J, April 1, 2001; 15(6): 958 - 969.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. J. Pan, J. Hancock, Z. Ding, D. Fogt, M. Lee, and J. L. Ivy
Effects of clenbuterol on insulin resistance in conscious obese Zucker rats
Am J Physiol Endocrinol Metab, April 1, 2001; 280(4): E554 - E561.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J.-M. Ye, P. J. Doyle, M. A. Iglesias, D. G. Watson, G. J. Cooney, and E. W. Kraegen
Peroxisome Proliferator--Activated Receptor (PPAR)-{alpha} Activation Lowers Muscle Lipids and Improves Insulin Sensitivity in High Fat--Fed Rats: Comparison With PPAR-{gamma} Activation
Diabetes, February 1, 2001; 50(2): 411 - 417.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. E. Halseth, D. P. Bracy, and D. H. Wasserman
Limitations to basal and insulin-stimulated skeletal muscle glucose uptake in the high-fat-fed rat
Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1064 - E1071.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. S. Bell, C. Schmitz-Peiffer, M. Lim-Fraser, T. J. Biden, G. J. Cooney, and E. W. Kraegen
Acute reversal of lipid-induced muscle insulin resistance is associated with rapid alteration in PKC-theta localization
Am J Physiol Endocrinol Metab, November 1, 2000; 279(5): E1196 - E1201.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. A. Ellis, A. Poynten, A. J. Lowy, S. M. Furler, D. J. Chisholm, E. W. Kraegen, and G. J. Cooney
Long-chain acyl-CoA esters as indicators of lipid metabolism and insulin sensitivity in rat and human muscle
Am J Physiol Endocrinol Metab, September 1, 2000; 279(3): E554 - E560.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. Zhou, G. Vallega, K. V. Kandror, and P. F. Pilch
Insulin-mediated translocation of GLUT-4-containing vesicles is preserved in denervated muscles
Am J Physiol Endocrinol Metab, June 1, 2000; 278(6): E1019 - E1026.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
J. M. Hernandez, M. J. Fedele, and P. A. Farrell
Time course evaluation of protein synthesis and glucose uptake after acute resistance exercise in rats
J Appl Physiol, March 1, 2000; 88(3): 1142 - 1149.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
I. Azpiazu, J. Manchester, A. V. Skurat, P. J. Roach, and J. C. Lawrence Jr.
Control of glycogen synthesis is shared between glucose transport and glycogen synthase in skeletal muscle fibers
Am J Physiol Endocrinol Metab, February 1, 2000; 278(2): E234 - E243.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
L. H. Enevoldsen, B. Stallknecht, J. D. Fluckey, and H. Galbo
Effect of exercise training on in vivo insulin-stimulated glucose uptake in intra-abdominal adipose tissue in rats
Am J Physiol Endocrinol Metab, January 1, 2000; 278(1): E25 - E34.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. J. Pagliassotti, E. C. Gayles, D. A. Podolin, Y. Wei, and C. L. Morin
Developmental stage modifies diet-induced peripheral insulin resistance in rats
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2000; 278(1): R66 - R73.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. R. Laybutt, C. Schmitz-Peiffer, A. K. Saha, N. B. Ruderman, T. J. Biden, and E. W. Kraegen
Muscle lipid accumulation and protein kinase C activation in the insulin-resistant chronically glucose-infused rat
Am J Physiol Endocrinol Metab, December 1, 1999; 277(6): E1070 - E1076.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. Bergeron, R. R. Russell III, L. H. Young, J.-M. Ren, M. Marcucci, A. Lee, and G. I. Shulman
Effect of AMPK activation on muscle glucose metabolism in conscious rats
Am J Physiol Endocrinol Metab, May 1, 1999; 276(5): E938 - E944.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. J. Wetter, A. C. Gazdag, D. J. Dean, and G. D. Cartee
Effect of calorie restriction on in vivo glucose metabolism by individual tissues in rats
Am J Physiol Endocrinol Metab, April 1, 1999; 276(4): E728 - E738.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. Zhou, L. Sevilla, G. Vallega, P. Chen, M. Palacin, A. Zorzano, P. F. Pilch, and K. V. Kandror
Insulin-dependent protein trafficking in skeletal muscle cells
Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E187 - E196.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J.-Y. Park, C.-H. Kim, S. K. Hong, K. I. Suh, and K.-U. Lee
Effects of FFA on insulin-stimulated glucose fluxes and muscle glycogen synthase activity in rats
Am J Physiol Endocrinol Metab, August 1, 1998; 275(2): E338 - E344.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
X. Liu, F. Perusse, and L. J. Bukowiecki
Mechanisms of the antidiabetic effects of the beta 3-adrenergic agonist CL-316243 in obese Zucker-ZDF rats
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 1998; 274(5): R1212 - R1219.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. Roy, M. Perreault, and A. Marette
Insulin stimulation of glucose uptake in skeletal muscles and adipose tissues in vivo is NO dependent
Am J Physiol Endocrinol Metab, April 1, 1998; 274(4): E692 - E699.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. M. O'Doherty, A. E. Halseth, D. K. Granner, D. P. Bracy, and D. H. Wasserman
Analysis of insulin-stimulated skeletal muscle glucose uptake in conscious rat using isotopic glucose analogs
Am J Physiol Endocrinol Metab, February 1, 1998; 274(2): E287 - E296.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
R. O. Deems, R. C. Anderson, and J. E. Foley
Hypoglycemic effects of a novel fatty acid oxidation inhibitor in rats and monkeys
Am J Physiol Regulatory Integrative Comp Physiol, February 1, 1998; 274(2): R524 - R528.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. Hettiarachchi, S. Chalkley, S. M. Furler, Y.-S. Choong, M. Heller, G. J. S. Cooper, and E. W. Kraegen
Rat amylin-(8---37) enhances insulin action and alters lipid metabolism in normal and insulin-resistant rats
Am J Physiol Endocrinol Metab, November 1, 1997; 273(5): E859 - E867.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
P. A. Hansen, D. H. Han, L. A. Nolte, M. Chen, and J. O. Holloszy
DHEA protects against visceral obesity and muscle insulin resistance in rats fed a high-fat diet
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 1997; 273(5): R1704 - R1708.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Rossetti, D. Massillon, N. Barzilai, P. Vuguin, W. Chen, M. Hawkins, J. Wu, and J. Wang
Short Term Effects of Leptin on Hepatic Gluconeogenesis and in Vivo Insulin Action
J. Biol. Chem., October 31, 1997; 272(44): 27758 - 27763.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
J. W. Slot, G. Garruti, S. Martin, V. Oorschot, G. Posthuma, E. W. Kraegen, R. Laybutt, G. Thibault, and D. E. James
Glucose Transporter (GLUT-4) Is Targeted to Secretory Granules in Rat Atrial Cardiomyocytes
J. Cell Biol., June 16, 1997; 137(6): 1243 - 1254.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online