AJP - Endocrinology and Metabolism, Vol 246, Issue 1 52-E61, Copyright © 1984 by American Physiological Society
Modeling glucose disposal in diabetic dogs fed mixed meals
Y. Yamasaki, J. Tiran and A. M. Albisser
We have utilized a previously described mathematical model to study glucose
disposal in fed, conscious, ambulatory, diabetic dogs. The model was
applied to estimate the daily disposition of ingested glucose in the
periphery, liver, and urine following a regular mixed meal containing 130 g
of carbohydrate. Experimental data was obtained from 11 pancreatectomized
animals. Both the portal and peripheral routes were used for intravenous
insulin infusion and the daily profiles of peripheral plasma glucose and
insulin concentrations measured. Total calories in mixed meals derived from
carbohydrates (37%), fat (30%), and protein (30%). When judged according to
the root-mean-square differences, agreement was excellent between
model-predicted and experimentally observed glucose as well as insulin
concentrations. This agreement occurred whether or not, in addition to
basal insulin, meal insulin was also given. Using the model, we then
predicted in detail the rates of glucose uptake in peripheral tissue,
liver, and kidneys. With portally infused insulin resulting in diurnal
glycemic normalization, the net daily hepatic glucose balance was
physiological, being close to zero. Remarkably, with peripheral insulin
infusions there was an unphysiological net negative hepatic glucose balance
of 10 g/day.
