AJP - Endocrinology and Metabolism, Vol 245, Issue 4 313-E317, Copyright © 1983 by American Physiological Society
Effects of PHI on hormonal secretion from perfused rat pancreas
J. Szecowka, D. Tendler and S. Efendic
Effects of the novel gastrointestinal polypeptide PHI with N-terminal
histidine, C-terminal isoleucine amide, and 27 amino acids have been
studied in isolated perfused rat pancreas. PHI increased the release of
insulin, glucagon, and somatostatin. The amounts of these hormones released
were strictly dependent on the prevailing glucose concentrations. In the
absence of glucose, PHI (1 nmol/liter) stimulated glucagon release. In the
presence of 4.4 and 6.7 mmol/liter glucose, the same dose of this peptide
stimulated insulin and somatostatin release. In the presence of 16.7
mmol/liter glucose, only insulin secretion was increased by PHI. When
arginine was used as a secretagogue, PHI (10 nmol/liter) potentiated
secretion of insulin, glucagon, and somatostatin. Thus, PHI may take part
in the regulation of the function of the pancreatic A, B, and D cells.
