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Am J Physiol Endocrinol Metab 243: E352-E359, 1982;
0193-1849/82 $5.00
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AJP - Endocrinology and Metabolism, Vol 243, Issue 5 352-E359, Copyright © 1982 by American Physiological Society

ARTICLES

Isolated perfused Brockmann body as a model for studying pancreatic endocrine secretion

P. Ronner and A. Scarpa

The release of insulin, glucagon, and somatostatin was investigated in a newly developed in vitro perfusion preparation of the splenic Brockmann body (principal pancreatic islet) of the channel catfish (Ictalurus punctatus). The preparation was viable for up to 7 h by functional and morphological criteria. Glucose evoked a biphasic release of insulin and somatostatin, but had no effect on the release of glucagon. Arginine at basal glucose concentration caused a marked release of insulin and glucagon, but only a minor release of somatostatin.




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