AJP - Endocrinology and Metabolism, Vol 240, Issue 6 609-E614, Copyright © 1981 by American Physiological Society
Prostaglandins and renin release in vitro
C. S. Lin, H. Iwao, S. Puttkammer and A. M. Michelakis
The present studies were undertaken to explore further the role of
prostaglandins in the release of renin from the renal cortex. To provide
the best assessment of renin release, renin was determined by a
radioimmunoassay for the direct measurement of renin. Slices of mouse renal
cortex were incubated at 37 degrees C with arachidonic acid (AA),
5,8,11,14-eicosatetraenoic acid (ETA), indomethacin, prostaglandins, and
synthetic prostaglandin endoperoxide analogue (EPA). Our results showed
that AA at 1.5 X 10(-8) M significantly increased renin release at 10 and
30 min of incubation. This renin increase ws abolished by either ETA or
indomethacin. Prostaglandin F2 alpha (PGF2 alpha) also significantly
stimulated renin release at 10 and 60 min. PGE2 and 16,16-dimethyl PGE2
(DMPGE2) showed much less renin release-stimulating activity. EPA and PGI2
on the other hand very strongly stimulated renin release. However, at
higher concentrations the stimulating effect of PGI2 and EPA disappeared
and even became inhibitory in the case of EPA. Other prostaglandins were
found to have no effect on renin release. The results suggest that the
prostaglandin system directly affects renin release from the
juxtaglomerular cells independent of systemic neurogenic and hemodynamic
influences.
