AJP - Endocrinology and Metabolism, Vol 238, Issue 2 150-E156, Copyright © 1980 by American Physiological Society
Insulin, glucagon, and somatostatin secretion from isolated perfused rat and chicken pancreas-duodenum
R. N. Honey, J. A. Schwarz, C. J. Mathe and G. C. Weir
Insulin, glucagon, and somatostatin secretion were evaluated in the
following isolated perfused models: rat pancreas-duodenum (both normal and
streptozotocin-diabetic animals) and the chicken pancreas with and without
duodenum. Insulin secretion in response to glucose or arginine was greater
from the normal rat than either the diabetic rat or the chicken. Glucagon
release from both species was suppressed by glucose and stimulated by
arginine except that poor inhibition by glucose was found in the diabetic
rat. Somatostatin could be measured in the effluent from both normal and
diabetic rats, but the responses to glucose and arginine were variable and
modest. Clear increases of secretion in the rat were only observed in
response to a combination of glucose, arginine, theophylline, and
isoproterenol. In contrast, the chicken somatostatin secretion was markedly
stimulated by glucose and by arginine. In conclusion, the perfused chicken
pancreas-duodenum has been shown to secrete large amounts of somatostatin
in comparison to the rat and should prove to be a useful system for the
study of D-cell regulation.
